| Title: | Phosphorylation of FAR1 in response to alpha-factor: a possible requirement for cell-cycle arrest |
| Address: | "Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448" |
| ISSN/ISBN: | 1059-1524 (Print) 1059-1524 (Linking) |
| Abstract: | "Exposure of yeast a cells to alpha-factor causes cells to arrest in the G1 phase of the cell cycle. The FAR1 gene is required for this cell-cycle arrest; its product is necessary for the inhibition of a G1 cyclin, CLN2. Earlier work demonstrated that alpha-factor caused an increase in the transcription of FAR1 severalfold over a measurable basal level. We now show that transcriptional induction of FAR1 from a heterologous promoter is not sufficient to inhibit CLN2 in the absence of alpha-factor. We also show that FAR1 is phosphorylated in response to alpha-factor and propose that this phosphorylation may be required for FAR1 activity" |
| Keywords: | "Base Sequence Cell Cycle/drug effects/*physiology Fungal Proteins/metabolism *Gene Expression Regulation, Fungal Genes, Fungal Mating Factor Molecular Sequence Data Peptides/*pharmacology Pheromones/*pharmacology Phosphorylation Promoter Regions, Genetic;" |
| Notes: | "MedlineChang, F Herskowitz, I eng GM-31286/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1992/04/01 Mol Biol Cell. 1992 Apr; 3(4):445-50. doi: 10.1091/mbc.3.4.445" |
