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FEMS Microbiol Lett

Title:		Molecular characterization of Shewanella and Aeromonas isolates associated with spoilage of Common carp (Cyprinus carpio)
Author(s):		Beaz-Hidalgo R; Agueria D; Latif-Eugenin F; Yeannes MI; Figueras MJ;
Address:		"Unitat de Microbiologia. Departament de Ciences Mediques Basiques, Facultat de Medicina i Ciencies de la Salut. IISPV. Universitat Rovira i Virgili, Sant Lorenzo 21, 43201, Reus, Spain. Departamento de Tecnologia y Calidad de los Alimentos, Facultad Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires B700, Argentina Instituto Multidisciplinario sobre Ecosistemas y Desarrollo Sustentable, Universidad Nacional del Centro de la Provincia de Buenos Aires B7000 Tandil, Buenos Aires, Argentina. Grupo de Investigacion Preservacion y Calidad de Alimentos. Departamento de Ingenieria Quimica. Facultad de Ingenieria, Universidad Nacional de Mar del Plata 7600 Mar del Plata, Buenos Aires, Argentina Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), 7600 Mar del Plata, Buenos Aires, Argentina. Unitat de Microbiologia. Departament de Ciences Mediques Basiques, Facultat de Medicina i Ciencies de la Salut. IISPV. Universitat Rovira i Virgili, Sant Lorenzo 21, 43201, Reus, Spain mariajose.figueras@urv.cat"
Journal Title:		FEMS Microbiol Lett
Year:		2015
Volume:		20141204
Issue:		1
Page Number:		1 - 8
DOI:		10.1093/femsle/fnu029
ISSN/ISBN:		1574-6968 (Electronic) 0378-1097 (Linking)
Abstract:		"Storage in ice is a common way of preserving commercial fish species but some microorganisms can still contaminate and participate in the spoilage of the product; therefore, identification of potential harmful microbes is important. Thirteen colonies were isolated from common carp (Cyprinus carpio) that had been stored in ice, whose phenotypic identification revealed that they belonged to the genera Aeromonas (n = 5) and Shewanella (n = 8). Molecular genotyping with ERIC-PCR showed clonality only among two of the five Aeromonas isolates and for two groups (n = 3; n = 2) of the eight Shewanella isolates. Sequencing the rpoD gene showed that four Aeromonas isolates belonged to the species Aeromonas salmonicida and one to A. sobria. Of the eight Shewanella, seven isolates cluster with Shewanella putrefaciens and one with Shewanella profunda in the 16S rRNA phylogenetic tree. However, analysis of the gyrB gene showed that these eight isolates could constitute a new species closely related to S. baltica. The Shewanella and A. salmonicida isolates produce off-odours and reduce trimethylamine oxide, indicating that they might contribute to the spoilage of the fish"
Keywords:		"Aeromonas/*classification/genetics/*isolation & purification/metabolism Animals Carps/*microbiology Cluster Analysis DNA Gyrase/genetics DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics DNA-Directed RNA Polymerases/genetics Food Microbi;"
Notes:		"MedlineBeaz-Hidalgo, Roxana Agueria, Daniela Latif-Eugenin, Fadua Yeannes, Maria I Figueras, Maria J eng Research Support, Non-U.S. Gov't England 2015/03/20 FEMS Microbiol Lett. 2015 Jan; 362(1):1-8. doi: 10.1093/femsle/fnu029. Epub 2014 Dec 4"