| Title: | Anesthetic action of volatile anesthetics by using Paramecium as a model |
| Author(s): | Zhou M; Xia H; Xu Y; Xin N; Liu J; Zhang S; |
| Address: | "Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. zhang_shihai@yahoo.cn" |
| Journal Title: | J Huazhong Univ Sci Technolog Med Sci |
| DOI: | 10.1007/s11596-012-0071-1 |
| ISSN/ISBN: | 1672-0733 (Print) 1672-0733 (Linking) |
| Abstract: | "Although empirically well understood in their clinical administration, volatile anesthetics are not yet well comprehended in their mechanism studies. A major conundrum emerging from these studies is that there is no validated model to assess the presumed candidate sites of the anesthetics. We undertook this study to test the hypothesis that the single-celled Paramecium could be anesthetized and served as a model organism in the study of anesthetics. We assessed the motion of Paramecium cells with Expert Vision system and the chemoresponse of Paramecium cells with T-maze assays in the presence of four different volatile anesthetics, including isoflurane, sevoflurane, enflurane and ether. Each of those volatiles was dissolved in buffers to give drug concentrations equal to 0.8, 1.0, and 1.2 EC50, respectively, in clinical practice. We could see that after application of volatile anesthetics, the swimming of the Paramecium cells was accelerated and then suppressed, or even stopped eventually, and the index of the chemoresponse of the Paramecium cells (denoted as I ( che )) was decreased. All of the above impacts were found in a concentration-dependent fashion. The biphasic effects of the clinical concentrations of volatile anesthetics on Paramecium simulated the situation of high species in anesthesia, and the inhibition of the chemoresponse also indicated anesthetized. In conclusion, the findings in our studies suggested that the single-celled Paramecium could be anesthetized with clinical concentrations of volatile anesthetics and therefore be utilized as a model organism to study the mechanisms of volatile anesthetics" |
| Keywords: | "Anesthetics, Inhalation/*administration & dosage Biological Assay/*methods Cell Movement/*drug effects/physiology Chemotaxis/*drug effects/physiology Dose-Response Relationship, Drug Drug Evaluation, Preclinical/methods Paramecium tetraurelia/*drug effect;" |
| Notes: | "MedlineZhou, Miaomiao Xia, Huimin Xu, Younian Xin, Naixing Liu, Jiao Zhang, Shihai eng Research Support, Non-U.S. Gov't China 2012/06/12 J Huazhong Univ Sci Technolog Med Sci. 2012 Jun; 32(3):410-414. doi: 10.1007/s11596-012-0071-1. Epub 2012 Jun 9" |
