| Title: | Docosahexaenoic acid inhibits pheromone-responsive-plasmid-mediated conjugative transfer of antibiotic resistance genes in Enterococcus faecalis |
| Author(s): | Zhou H; Yang X; Yang Y; Niu Y; Li J; Fu X; Wang S; Xue B; Li C; Zhao C; Zhang X; Shen Z; Wang J; Qiu Z; |
| Address: | "Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China. Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China; Shanghai Ocean University, Shanghai 201306, China. Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China; Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China. Electronic address: zhigangqiu99@gmail.com" |
| DOI: | 10.1016/j.jhazmat.2022.130390 |
| ISSN/ISBN: | 1873-3336 (Electronic) 0304-3894 (Linking) |
| Abstract: | "The rapid spread of antibiotic-resistance genes (ARGs) in Enterococcus faecalis (E. faecalis) poses a great challenge to human health and ecological and environmental safety. Therefore, it is important to control the spread of ARGs. In this study, we observed that the addition of 5 mug/mL docosahexaenoic acid (DHA) reduced the conjugative transfer of pCF10 plasmid by more than 95% in E. faecalis. DHA disturbed the pheromone transport by inhibiting the mRNA levels of the prgZ gene, causing the iCF10 pheromone to accumulate in the donor bacteria and bond to the PrgX receptor to form an inhibitory phase, which resulted in the down-regulation of the expression of genes related to conjugative transfer, inhibiting biofilm formation, reducing bacterial adhesion and thus inhibiting conjugative transfer. Collectively, DHA exhibited an admirable inhibitory effect on the transfer of ARGs in E. faecalis. This study provided a technical option to control the transfer of ARGs" |
| Keywords: | "Humans *Enterococcus faecalis/genetics *Docosahexaenoic Acids/pharmacology Anti-Bacterial Agents Pheromones Drug Resistance, Microbial Plasmids/genetics Antibiotic-resistance gene/ Conjugative transfer/ Docosahexaenoic acid/ Enterococcus faecalis/ Pheromo;" |
| Notes: | "MedlineZhou, Hongrui Yang, Xiaobo Yang, Yutong Niu, Yuanyuan Li, Jing Fu, Xinyue Wang, Shang Xue, Bin Li, Chenyu Zhao, Chen Zhang, Xi Shen, Zhiqiang Wang, Jingfeng Qiu, Zhigang eng Research Support, Non-U.S. Gov't Netherlands 2022/11/25 J Hazard Mater. 2023 Feb 15; 444(Pt A):130390. doi: 10.1016/j.jhazmat.2022.130390. Epub 2022 Nov 11" |
