| Title: | Enantiomeric separation and precise quantification of chiral volatiles in Wuyi rock teas using an efficient enantioselective GC x GC-TOFMS approach |
| Author(s): | Zhang Y; Zhang YH; Yan H; Shao CY; Li WX; Lv HP; Lin Z; Zhu Y; |
| Address: | "Key Laboratory of Tea Biology and Resource Untilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China. Key Laboratory of Tea Biology and Resource Untilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China. Key Laboratory of Tea Biology and Resource Untilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China. Electronic address: linzhi@caas.cn. Key Laboratory of Tea Biology and Resource Untilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China. Electronic address: zhuy_scu@tricaas.com" |
| DOI: | 10.1016/j.foodres.2023.112891 |
| ISSN/ISBN: | 1873-7145 (Electronic) 0963-9969 (Linking) |
| Abstract: | "Chiral volatiles play important roles in the formation of aroma quality of foods. To date, enantiomeric characteristics of chiral volatiles in Wuyi rock tea (WRT) and their aroma contributions are still unclear. In this study, an efficient enantioselective comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (Es-GC x GC-TOFMS) approach to separate and precisely quantitate 24 pairs of chiral volatiles in WRTs was established, and the enantiomeric distribution and aroma contribution of chiral volatiles among WRTs from four representative cultivars were investigated. Enantiomeric ratio (ER) of R-alpha-ionone (80%) in Dahongpao (DHP), ER of S-alpha-terpineol (57%) in Jinfo (JF), ERs of R-gamma-heptanolactone (69%), S-gamma-nonanolactone (55%), (2R, 5S)-theaspirane B (91%), concentration of S-(E)-nerolidol (313.37 ng/mL) in Rougui (RG) and concentration of R-alpha-ionone (33.01 ng/mL) in Shuixian (SX) were unique from other types of WRTs, which were considered as the potential chemical markers to distinguish WRT cultivars. The OAV assessment determined 7 volatile enantiomers as the aroma-active compounds, especially R-alpha-ionone and R-delta-octanolactone in SX, as well as S-(E)-nerolidol and (1R, 2R)-methyl jasmonate in RG contribute much to aroma formation of the corresponding WRTs. The above results provide scientific references for discrimination of tea cultivars and directed improvement of the aroma quality of WRT" |
| Keywords: | *Tea/chemistry Stereoisomerism *Volatile Organic Compounds/analysis Chiral volatiles Cultivars Es-GC x GC-TOFMS OAV calculation Wuyi rock teas; |
| Notes: | "MedlineZhang, Yue Zhang, Yu-Hui Yan, Han Shao, Chen-Yang Li, Wei-Xuan Lv, Hai-Peng Lin, Zhi Zhu, Yin eng Research Support, Non-U.S. Gov't Canada 2023/05/31 Food Res Int. 2023 Jul; 169:112891. doi: 10.1016/j.foodres.2023.112891. Epub 2023 Apr 30" |
