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Insect Sci

Title:		A novel domain-duplicated SlitFAR3 gene involved in sex pheromone biosynthesis in Spodoptera litura
Author(s):		Zhang B; Li F; Qu C; Duan H; Fu Y; Luo C;
Address:		"Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, China. Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China. Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, China"
Journal Title:		Insect Sci
Year:		2023
Volume:		20221130
Issue:		3
Page Number:		611 - 624
DOI:		10.1111/1744-7917.13131
ISSN/ISBN:		1744-7917 (Electronic) 1672-9609 (Linking)
Abstract:		"Fatty acyl reductases (FARs) are key enzymes that participate in sex pheromone biosynthesis by reducing fatty acids to fatty alcohols. Lepidoptera typically harbor numerous FAR gene family members. Although FAR genes are involved in the biosynthesis of sex pheromones in moths, the key FAR gene of Spodoptera litura remains unclear. In this work, we predicted 30 FAR genes from the S. litura genome and identified a domain duplication within gene SlitFAR3, which exhibited high and preferential expression in the sexually mature female pheromone glands (PGs) and a rhythmic expression pattern during the scotophase of sex pheromone production. The molecular docking of SlitFAR3, as predicted using a 3D model, revealed a co-factor NADPH binding cavity and 2 substrate binding cavities. Functional expression in yeast cells combined with comprehensive gas chromatography indicated that the SlitFAR3 gene could produce fatty alcohol products. This study is the first to focus on the special phenomenon of FAR domain duplication, which will advance our understanding of biosynthesis-related genes from the perspective of evolutionary biology"
Keywords:		Female Animals *Sex Attractants/metabolism Spodoptera/genetics/metabolism Amino Acid Sequence Molecular Docking Simulation *Moths/genetics SlitFAR3 Spodoptera litura domain duplication fatty acyl reductase molecular docking yeast expression;
Notes:		"MedlineZhang, Biyun Li, Fengqi Qu, Cheng Duan, Hongxia Fu, Yuejun Luo, Chen eng 2019YFD1002100/National Key R & D Program of China/ CARS-24-C-03/China Agriculture Research System of MOF and MARA/ Australia 2022/10/28 Insect Sci. 2023 Jun; 30(3):611-624. doi: 10.1111/1744-7917.13131. Epub 2022 Nov 30"