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Front Microbiol

Title:		Multiple Modes of Nematode Control by Volatiles of Pseudomonas putida 1A00316 from Antarctic Soil against Meloidogyne incognita
Author(s):		Zhai Y; Shao Z; Cai M; Zheng L; Li G; Huang D; Cheng W; Thomashow LS; Weller DM; Yu Z; Zhang J;
Address:		"State Key Laboratory of Agricultural Microbiology and National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China. Wheat Health, Genetics and Quality Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA, United States"
Journal Title:		Front Microbiol
Year:		2018
Volume:		20180223
Issue:
Page Number:		253 -
DOI:		10.3389/fmicb.2018.00253
ISSN/ISBN:		1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking)
Abstract:		"Pseudomonas putida 1A00316 isolated from Antarctic soil showed nematicidal potential for biological control of Meloidogyne incognita; however, little was known about whether strain 1A00316 could produce volatile organic compounds (VOCs), and if they had potential for use in biological control against M. incognita. In this study, VOCs produced by a culture filtrate of P. putida 1A00316 were evaluated by in vitro experiments in three-compartment Petri dishes and 96-well culture plates. Our results showed that M. incognita juveniles gradually reduced their movement within 24-48 h of incubation with mortality ranging from 6.49 to 86.19%, and mostly stopped action after 72 h. Moreover, egg hatching in culture filtrates of strain 1A00316 was much reduced compared to that in sterile distilled water or culture medium. Volatiles from P. putida 1A00316 analysis carried out by solid-phase micro-extraction gas chromatography-mass spectrometry (SPME-GC/MS) included dimethyl-disulfide, 1-undecene, 2-nonanone, 2-octanone, (Z)-hexen-1-ol acetate, 2-undecanone, and 1-(ethenyloxy)-octadecane. Of these, dimethyl-disulfide, 2-nonanone, 2-octanone, (Z)-hexen-1-ol acetate, and 2-undecanone had strong nematicidal activity against M. incognita J2 larvae by direct-contact in 96-well culture plates, and only 2-undecanone acted as a fumigant. In addition, the seven VOCs inhibited egg hatching of M. incognita both by direct-contact and by fumigation. All of the seven VOCs repelled M. incognita J2 juveniles in 2% water agar Petri plates. These results show that VOCs from strain 1A00316 act on different stages in the development of M. incognita via nematicidal, fumigant, and repellent activities and have potential for development as agents with multiple modes of control of root-knot nematodes"
Keywords:		Meloidogyne incognita Pseudomonas putida 1A00316 chemotaxis egg hatching nematode control volatile organic compound;
Notes:		"PubMed-not-MEDLINEZhai, Yile Shao, Zongze Cai, Minmin Zheng, Longyu Li, Guangyu Huang, Dian Cheng, Wanli Thomashow, Linda S Weller, David M Yu, Ziniu Zhang, Jibin eng Switzerland 2018/03/31 Front Microbiol. 2018 Feb 23; 9:253. doi: 10.3389/fmicb.2018.00253. eCollection 2018"