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JPEN J Parenter Enteral Nutr

Title:		Microbiome and Metabolome Profiles Associated With Different Types of Short Bowel Syndrome: Implications for Treatment
Author(s):		Budinska E; Gojda J; Heczkova M; Bratova M; Dankova H; Wohl P; Bastova H; Lanska V; Kostovcik M; Dastych M; Senkyrik M; Krizova J; Mraz M; Hradecky J; Hajslova J; Lenicek M; Podzimkova K; Chalupsky K; Sedlacek R; Cahova M;
Address:		"RECETOX, Faculty of Science Masaryk University, Brno, Czech Republic. 2nd Department of Internal Medicine, Kralovske Vinohrady University Hospital and 3rd Faculty of Medicine, Charles University, Prague, Czech Republic. Institute for Clinical and Experimental Medicine, Prague, Czech Republic. BIOCEV, Institute of Microbiology, AS CR, Vestec, Czech Republic. Department of Gastroenterology and Internal Medicine, University Hospital Brno, Brno, Czech Republic. 3rd Department of Medicine, Department of Endocrinology and Metabolism, General University Hospital and 1st Faculty of Medicine, Charles University, Prague, Czech Republic. University of Chemistry and Technology, Prague, Czech Republic. Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Prague, Czech Republic. Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University, Prague, Czech Republic. Czech Centre for Phenogenomics, Vestec, Czech Republic. Laboratory of Transgenic Diseases, Institute of Molecular Genetics CAS, Prague, Czech Republic"
Journal Title:		JPEN J Parenter Enteral Nutr
Year:		2020
Volume:		20190429
Issue:		1
Page Number:		105 - 118
DOI:		10.1002/jpen.1595
ISSN/ISBN:		1941-2444 (Electronic) 0148-6071 (Linking)
Abstract:		"BACKGROUND: The gut microbiome and metabolome may significantly influence clinical outcomes in patients with short bowel syndrome (SBS). The study aimed to describe specific metagenomic/metabolomics profiles of different SBS types and to identify possible therapeutic targets. METHODS: Fecal microbiome (FM), volatile organic compounds (VOCs), and bile acid (BA) spectrum were analyzed in parenteral nutrition (PN)-dependent SBS I, SBS II, and PN-independent (non-PN) SBS patients. RESULTS: FM in SBS I, SBS II, and non-PN SBS shared characteristic features (depletion of beneficial anaerobes, high abundance of Lactobacilaceae and Enterobacteriaceae). SBS I patients were characterized by the abundance of oxygen-tolerant microrganisms and depletion of strict anaerobes. Non-PN SBS subjects showed markers of partial FM normalization. FM dysbiosis was translated into VOC and BA profiles characteristic for each SBS cohort. A typical signature of all SBS patients comprised high saturated aldehydes and medium-chain fatty acids and reduced short-chain fatty acid (SCFA) content. Particularly, SBS I and II exhibited low protein metabolism intermediate (indole, p-cresol) content despite the hypothetical presence of relevant metabolism pathways. Distinctive non-PN SBS marker was high phenol content. SBS patients' BA fecal spectrum was enriched by chenodeoxycholic and deoxycholic acids and depleted of lithocholic acid. CONCLUSIONS: Environmental conditions in SBS gut significantly affect FM composition and metabolic activity. The common feature of diverse SBS subjects is the altered VOC/BA profile and the lack of important products of microbial metabolism. Strategies oriented on the microbiome/metabolome reconstitution and targeted delivery of key compounds may represent a promising therapeutic strategy in SBS patients"
Keywords:		Bacteria/*classification Bile Acids and Salts/analysis Dysbiosis Feces/microbiology *Gastrointestinal Microbiome Humans *Metabolome Parenteral Nutrition Short Bowel Syndrome/*microbiology Volatile Organic Compounds/analysis bile acids gut microbiota short;
Notes:		"MedlineBudinska, Eva Gojda, Jan Heczkova, Marie Bratova, Miriam Dankova, Helena Wohl, Petr Bastova, Hana Lanska, Vera Kostovcik, Martin Dastych, Milan Senkyrik, Michal Krizova, Jarmila Mraz, Milos Hradecky, Jaromir Hajslova, Jana Lenicek, Martin Podzimkova, Katerina Chalupsky, Karel Sedlacek, Radislav Cahova, Monika eng Research Support, Non-U.S. Gov't 2019/04/30 JPEN J Parenter Enteral Nutr. 2020 Jan; 44(1):105-118. doi: 10.1002/jpen.1595. Epub 2019 Apr 29"