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Folia Microbiol (Praha)

Title:		Effect of inactivation of luxS gene on the properties of Serratia proteamaculans 94 strain
Author(s):		Zaitseva YV; Lipasova VA; Plyuta VA; Koksharova OA; Demidyuk IV; Kostrov SV; Khmel IA;
Address:		"Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq. 2, Moscow, Russia, 123182. Demidov Yaroslavl State University, Sovetskaya Str. 14, Yaroslavl, Russia, 150003. Lomonosov Moscow State University, Belozersky Institute of Physical-Chemical Biology, Leninskie Gory, 1-40, Moscow, Russia, 119992. Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq. 2, Moscow, Russia, 123182. khmel@img.ras.ru"
Journal Title:		Folia Microbiol (Praha)
Year:		2019
Volume:		20181025
Issue:		3
Page Number:		265 - 272
DOI:		10.1007/s12223-018-0657-5
ISSN/ISBN:		1874-9356 (Electronic) 0015-5632 (Linking)
Abstract:		"The luxS gene is responsible for the synthesis of AI-2 (autoinducer-2), a signaling molecule that participates in quorum sensing regulation in a large number of bacteria. In this work, we investigated which phenotypes are regulated by luxS gene in Serratia proteamaculans 94, psychrotrophic strain isolated from spoiled refrigerated meat. AI-2 was identified in S. proteamaculans 94, and the luxS gene involved in its synthesis was cloned and sequenced. A mutant with the inactivated luxS gene was constructed. Inactivation of the luxS gene was shown to lead to the absence of AI-2 synthesis, chitinolytic activity, swimming motility, suppression of the growth of fungal plant pathogens Rhizoctonia solani and Helminthosporium sativum by volatile compounds emitted by S. proteamaculans 94 strain, and to a decrease of extracellular proteolytic activity. The knockout of the luxS gene did not affect synthesis of N-acyl-homoserine lactones, lipolytic, and hemolytic activities of S. proteamaculans 94"
Keywords:		"Bacterial Proteins/*genetics Biofilms/growth & development Carbon-Sulfur Lyases/*genetics Gene Expression Regulation, Bacterial *Gene Silencing Homoserine/analogs & derivatives/metabolism Lactones/metabolism Meat/microbiology Microbial Interactions Phenot;"
Notes:		"MedlineZaitseva, Yu V Lipasova, V A Plyuta, V A Koksharova, O A Demidyuk, I V Kostrov, S V Khmel, I A eng 16-04-01048/Russian Foundation for Basic Research/ 18-04-00375/Russian Foundation for Basic Research/ 2018/10/27 Folia Microbiol (Praha). 2019 May; 64(3):265-272. doi: 10.1007/s12223-018-0657-5. Epub 2018 Oct 25"