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Front Physiol

Title:		Identification of Candidate Carboxylesterases Associated With Odorant Degradation in Holotrichia parallela Antennae Based on Transcriptome Analysis
Author(s):		Yi J; Wang S; Wang Z; Wang X; Li G; Zhang X; Pan Y; Zhao S; Zhang J; Zhou JJ; Wang J; Xi J;
Address:		"College of Plant Science, Jilin University, Changchun, China. School of Life Science, Huizhou University, Huizhou, China. Changchun Customs Technology Center, Changchun, China. Rothamsted Research, University of Hertfordshire, Harpenden, United Kingdom"
Journal Title:		Front Physiol
Year:		2021
Volume:		20210910
Issue:
Page Number:		674023 -
DOI:		10.3389/fphys.2021.674023
ISSN/ISBN:		1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking)
Abstract:		"Insects rely on their olfactory systems in antennae to recognize sex pheromones and plant volatiles in surrounding environments. Some carboxylesterases (CXEs) are odorant-degrading enzymes (ODEs), degrading odorant signals to protect the olfactory neurons against continuous excitation. However, there is no report about CXEs in Holotrichia parallela, one of the most major agricultural underground pests in China. In the present study, 20 candidate CXEs were identified based on transcriptome analysis of female and male antennae. Sequence alignments and phylogenetic analysis were performed to investigate the characterization of these candidate CXEs. The expression profiles of CXEs were compared by RT-qPCR analysis between olfactory and non-olfactory tissues of both genders. HparCXE4, 11, 16, 17, 18, 19, and 20 were antenna-biased expressed genes, suggesting their possible roles as ODEs. HparCXE6, 10, 11, 13, and 16 showed significantly higher expression profiles in male antennae, whereas HparCXE18 was expressed more in female antennae. This study highlighted candidate CXE genes linked to odorant degradation in antennae, and provided a useful resource for further work on the H. parallela olfactory mechanism and selection of target genes for integrative control of H. parallela"
Keywords:		Holotrichia parallela antenna-biased expression profile antennal transcriptome carboxylesterase odorant-degrading enzyme;
Notes:		"PubMed-not-MEDLINEYi, Jiankun Wang, Shang Wang, Zhun Wang, Xiao Li, Gongfeng Zhang, Xinxin Pan, Yu Zhao, Shiwen Zhang, Juhong Zhou, Jing-Jiang Wang, Jun Xi, Jinghui eng Switzerland 2021/09/28 Front Physiol. 2021 Sep 10; 12:674023. doi: 10.3389/fphys.2021.674023. eCollection 2021"