| Title: | Biocidal effects of volatile organic compounds produced by the myxobacterium Corrallococcus sp. EGB against fungal phytopathogens |
| Author(s): | Ye X; Chen Y; Ma S; Yuan T; Wu Y; Li Y; Zhao Y; Chen S; Zhang Y; Li L; Li Z; Huang Y; Cao H; Cui Z; |
| Address: | "Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture and Rural Affairs, College of Life Science of Nanjing Agricultural University, Nanjing, 210095, China. Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture and Rural Affairs, College of Life Science of Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: czl@njau.edu.cn. Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture and Rural Affairs, College of Life Science of Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: labc_7021@163.com" |
| ISSN/ISBN: | 1095-9998 (Electronic) 0740-0020 (Linking) |
| Abstract: | "Myxobacteria have excellent biocontrol activity against various phytopathogens due to their rich spectrum of secondary metabolites and active predatory characteristics. In this study, the mycelial growth of Fusarium oxysporum f. sp. cucumerinum (FOC) was found to be significantly inhibited by volatile compounds (VOCs) produced by Corallococcus sp. EGB. A total of 32 compounds were identified among the VOCs produced by strain EGB, of which isooctanol exhibited the highest antifungal activity, with dosages of 3.75 and 4.0 muL/plate being sufficient to suppress FOC and Penicillum digitatum, respectively. Isooctanol was found to damage the cell wall and cell membranes of FOC and P. digitatum. Apoptosis-like cell death of FOC and P. digitatum induced by isooctanol was observed subsequently due to the accumulation of reactive oxygen species (ROS). The transcription level of genes related to cell wall integrity (CWI) pathway and redox reactions were significantly upregulated by 15- to 40-fold, indicating the stress caused by isooctanol. Postharvest storage experiments showed that the disease severity of post-harvest oranges infected with P. digitatum could be significantly reduced by isooctanol at 114.2 muL/L" |
| Keywords: | Antifungal Agents/metabolism/*pharmacology Cell Wall/drug effects/metabolism Citrus sinensis/microbiology Food Storage Fusarium/drug effects/growth & development/metabolism Microbial Viability/drug effects Mycelium/drug effects/growth & development/metabo; |
| Notes: | "MedlineYe, Xianfeng Chen, Yang Ma, Shiyun Yuan, Tian Wu, Yaxuan Li, Yingxuan Zhao, Yuqiang Chen, Shuying Zhang, Yiwen Li, Liuyan Li, Zhoukun Huang, Yan Cao, Hui Cui, Zhongli eng England 2020/06/17 Food Microbiol. 2020 Oct; 91:103502. doi: 10.1016/j.fm.2020.103502. Epub 2020 Apr 9" |
