| Title: | Octopamine terminates sex pheromone biosynthesis by suppressing PBAN signal in moths |
| Author(s): | Yang Y; Ma X; Zhang L; Zhao W; Liu X; Li X; Du M; An S; |
| Address: | "State key Laboratory of Wheat and Maize Crop Science/Henan International Laboratory for Green Pest Control/College of Plant Protection, Henan Agricultural University, Zhengzhou, China. Agricultural and Rural Bureau of Xincai, Zhumadian, China" |
| ISSN/ISBN: | 1365-2583 (Electronic) 0962-1075 (Linking) |
| Abstract: | "The biosynthesis and termination of insect sex pheromones should be accurately regulated. In most moths, the biosynthesis and release of sex pheromones are regulated by a class of neuropeptides known as pheromone biosynthesis activating neuropeptides (PBANs). However, endogenous mechanisms underlying the termination of sex pheromone biosynthesis in moths remain elusive. In the present study, Helicoverpa armigera was employed as a model to investigate the role of octopamine (OA) in the inhibition of sex pheromone biosynthesis. Results demonstrated that the release of sex pheromones decreased with an increase in OA titres in older female moths. Moreover, OA treatment led to a significant decrease in sex pheromone production, female capability to attract male counterparts and subsequent female acceptance, indicating its inhibitory role in sex pheromone release. Subsequent qPCR and RNAi analyses revealed that OctbetaR was a key receptor of OA that regulated sex pheromone biosynthesis. In addition, the OA/OctbetaR signal suppressed intracellular Ca(2+) levels and attenuated PBAN-mediated increase in the enzyme activities of calcineurin and acetyl-CoA carboxylase as demonstrated by OA treatment and OctbetaR-RNAi. Altogether, these results revealed a mechanism underlying the inhibition of sex pheromone production by OA via suppression of PBAN signalling in moths" |
| Keywords: | Animals Calcineurin Female Male *Moths/genetics *Neuropeptides/genetics Octopamine *Sex Attractants Helicoverpa armigera OctbetaR pheromone biosynthesis activating neuropeptide (PBAN) sex pheromone; |
| Notes: | "MedlineYang, Yue Ma, Xingyu Zhang, Long Zhao, Wenli Liu, Xiaoguang Li, Xiang Du, Mengfang An, Shiheng eng Research Support, Non-U.S. Gov't England 2022/06/03 Insect Mol Biol. 2022 Oct; 31(5):647-658. doi: 10.1111/imb.12793. Epub 2022 Jun 21" |
