| Title: | Improvement of flavor profiles in Chinese rice wine by creating fermenting yeast with superior ethanol tolerance and fermentation activity |
| Author(s): | Yang Y; Xia Y; Lin X; Wang G; Zhang H; Xiong Z; Yu H; Yu J; Ai L; |
| Address: | "Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, PR China. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, 200235, PR China. Shanghai Jinfeng Wine Co., Ltd., Shanghai, 200120, PR China. Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, PR China. Electronic address: ailianzhong@hotmail.com" |
| DOI: | 10.1016/j.foodres.2018.03.036 |
| ISSN/ISBN: | 1873-7145 (Electronic) 0963-9969 (Linking) |
| Abstract: | "Producing alcoholic beverages with novel flavor are desirable for winemakers. We created fermenting yeast with superior ethanol tolerance and fermentation activity to improve the flavor profiles of Chinese rice wine. Strategies of ethanol domestication, ultraviolet mutagenesis (UV) and protoplast fusion were conducted to create yeast hybrids with excellent oenological characteristic. The obtained diploid hybrid F23 showed a cell viability of 6.2% under 25% ethanol, whereas its diploid parental strains could not survive under 20% ethanol. During Chinese rice wine-making, compared to diploid parents, F23 produced 7.07%-12.44% higher yield of ethanol. Flavor analysis indicated that the total content of flavor compounds in F23 wine was 19.99-26.55% higher than that of parent wines. Specifically, F23 exhibited higher capacity in producing 2-phenylethanol, short-chain and long-chain fatty-acid ethyl-ester than diploid parents. Compared to diploid parents, F23 introduced more flavor contributors with odor activity values (OAVs) above one to Chinese rice wine, and those contributors were found with higher OAVs. Based on principal component analysis (PCA), the flavor characteristic of F23 wine was similar to each of parent wine. Additionally, sensory evaluation showed that F23 wine was highly assessed for its intensive levels in fruit-aroma, alcohol-aroma and mouthfeel. Hybrid F23 not only displayed superior flavor production and oenological performance in making Chinese rice wine, but also could act as potential 'mixed-like' starter to enrich wine style and differentiation" |
| Keywords: | Adult Ethanol/*metabolism Female *Fermentation/genetics Food Microbiology/methods Genotype Humans Judgment Male Microbial Viability Middle Aged Mutagenesis Mutation Odorants/*analysis Olfactory Perception Oryza/*microbiology Phenotype Saccharomyces cerevi; |
| Notes: | "MedlineYang, Yijin Xia, Yongjun Lin, Xiangna Wang, Guangqiang Zhang, Hui Xiong, Zhiqiang Yu, Haiyan Yu, Jianshen Ai, Lianzhong eng Comparative Study Research Support, Non-U.S. Gov't Canada 2018/05/08 Food Res Int. 2018 Jun; 108:83-92. doi: 10.1016/j.foodres.2018.03.036. Epub 2018 Mar 13" |
