| Title: | Identification of Genes Involved in Chemoreception in Plutella xyllostella by Antennal Transcriptome Analysis |
| Author(s): | Yang S; Cao D; Wang G; Liu Y; |
| Address: | "Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, 241000, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. yangliu@ippcaas.cn" |
| DOI: | 10.1038/s41598-017-11646-7 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Perception of environmental and habitat cues is of significance for insect survival and reproduction. Odor detection in insects is mediated by a number of proteins in antennae such as odorant receptors (ORs), ionotropic receptors (IRs), odorant binding proteins (OBPs), chemosensory proteins (CSPs), sensory neuron membrane proteins (SNMPs) and odorant degrading enzymes. In this study, we sequenced and assembled the adult male and female antennal transcriptomes of a destructive agricultural pest, the diamondback moth Plutella xyllostella. In these transcriptomes, we identified transcripts belonging to 6 chemoreception gene families related to ordor detection, including 54 ORs, 16 IRs, 7 gustatory receptors (GRs), 15 CSPs, 24 OBPs and 2 SNMPs. Semi-quantitative reverse transcription PCR analysis of expression patterns indicated that some of these ORs and IRs have clear sex-biased and tissue-specific expression patterns. Our results lay the foundation for future characterization of the functions of these P. xyllostella chemosensory receptors at the molecular level and development of novel semiochemicals for integrated control of this agricultural pest" |
| Keywords: | Animals Arthropod Antennae/*drug effects Chemoreceptor Cells/*drug effects Female *Gene Expression Profiling Lepidoptera/*drug effects Male Odorants Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction; |
| Notes: | "MedlineYang, Shiyong Cao, Depan Wang, Guirong Liu, Yang eng Research Support, Non-U.S. Gov't England 2017/09/22 Sci Rep. 2017 Sep 20; 7(1):11941. doi: 10.1038/s41598-017-11646-7" |
