Bedoukian   RussellIPM   RussellIPM   Piezoelectric Micro-Sprayer


Home
Animal Taxa
Plant Taxa
Semiochemicals
Floral Compounds
Semiochemical Detail
Semiochemicals & Taxa
Synthesis
Control
Invasive spp.
References

Abstract

Guide

Alphascents
Pherobio
InsectScience
E-Econex
Counterpart-Semiochemicals
Print
Email to a Friend
Kindly Donate for The Pherobase

« Previous AbstractChanges in the volatile profile of Pinot noir wines caused by Patagonian Lactobacillus plantarum and Oenococcus oeni strains    Next AbstractMetabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage »

J Cell Biol


Title:Cell fusion during yeast mating requires high levels of a-factor mating pheromone
Author(s):Brizzio V; Gammie AE; Nijbroek G; Michaelis S; Rose MD;
Address:"Department of Molecular Biology, Princeton University, New Jersey 08544-1014, USA"
Journal Title:J Cell Biol
Year:1996
Volume:135
Issue:6 Pt 2
Page Number:1727 - 1739
DOI: 10.1083/jcb.135.6.1727
ISSN/ISBN:0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking)
Abstract:"During conjugation, two yeast cells fuse to form a single zygote. Cell fusion requires extensive remodeling of the cell wall, both to form a seal between the two cells and to remove the intervening material. The two plasma membranes then fuse to produce a continuous cytoplasm. We report the characterization of two cell fusion defective (Fus-) mutants, fus5 and fus8, isolated previously in our laboratory. Fluorescence and electron microscopy demonstrated that the fus5 and fus8 mutant zygotes were defective for cell wall remodeling/removal but not plasma membrane fusion. Strikingly, fus5 and fus8 were a specific; both mutations caused the mutant phenotype when present in the MATa parent but not in the MAT alpha parent. Consistent with an a-specific defect, the fus5 and fus8 mutants produced less a-factor than the isogenic wild-type strain. FUS5 and FUS8 were determined to be allelic to AXL1 and RAM1, respectively, two genes known to be required for biogenesis of a-factor. Several experiments demonstrated that the partial defect in a-factor production resulted in the Fus- phenotype. First, overexpression of a-factor in the fus mutants suppressed the Fus- defect. Second, matings to an MAT alpha partner supersensitive to mating pheromone (sst2 delta) suppressed the Fus- defect in trans. Finally, the gene encoding a-factor, MFA1, was placed under the control of a repressible promoter; reduced levels of wild-type a-factor caused an identical cell fusion defect during mating. We conclude that high levels of pheromone are required as one component of the signal for prezygotes to initiate cell fusion"
Keywords:"Alleles Escherichia coli/genetics Fungal Proteins/genetics/metabolism Gene Expression Regulation, Fungal/physiology Insulysin/genetics Lipoproteins/*genetics/*metabolism Metalloendopeptidases Microscopy, Electron Molecular Sequence Data Mutation/physiolog;"
Notes:"MedlineBrizzio, V Gammie, A E Nijbroek, G Michaelis, S Rose, M D eng R01 GM037739/GM/NIGMS NIH HHS/ GM37739/GM/NIGMS NIH HHS/ GM41223/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1996/12/01 J Cell Biol. 1996 Dec; 135(6 Pt 2):1727-39. doi: 10.1083/jcb.135.6.1727"

 
Back to top
 
Citation: El-Sayed AM 2024. The Pherobase: Database of Pheromones and Semiochemicals. <http://www.pherobase.com>.
© 2003-2024 The Pherobase - Extensive Database of Pheromones and Semiochemicals. Ashraf M. El-Sayed.
Page created on 05-12-2024