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FEMS Yeast Res

Title:		Characterization of alpha-factor pheromone and pheromone receptor genes of Ashbya gossypii
Author(s):		Wendland J; Dunkler A; Walther A;
Address:		"Carlsberg Laboratory, Yeast Biology, Valby, Copenhagen, Denmark. jww@crc.dk"
Journal Title:		FEMS Yeast Res
Year:		2011
Volume:		20110506
Issue:		5
Page Number:		418 - 429
DOI:		10.1111/j.1567-1364.2011.00732.x
ISSN/ISBN:		1567-1364 (Electronic) 1567-1356 (Linking)
Abstract:		"The genome of Ashbya gossypii contains homologs of most of the genes that are part of the Saccharomyces cerevisiae pheromone-signal transduction cascade. However, we currently lack understanding of a potential sexual cycle for this pre-whole genome duplication hemiascomycete. The sequenced strain bears three identical copies encoding MATa. We show that the syntenic A. gossypii homolog of MFalpha1 (AFL062w) does not encode a mature alpha-factor peptide, but identified another gene, AAR163c, which encodes a candidate alpha-specific mating pheromone and is thus reannotated as AgMFalpha2. The expression of the AgSTE2alpha-factor receptor in an Scste2 S. cerevisiae MATa strain resulted in dosage-dependent growth arrest upon exposure to A. gossypiialpha-factor, which indicated that the pheromone response was effectively coupled to the S. cerevisiae signal transduction cascade. Comparison of alpha-pheromones and alpha-pheromone receptors showed greater conservation between Eremothecium cymbalariae and S. cerevisiae than between A. gossypii and E. cymbalariae. We constructed A. gossypii strains deleted for the STE2 and STE3 pheromone receptors. These strains showed no phenotypic abnormalities and an ste2, ste3 double mutant is still able to sporulate. The deletion of STE12 as the downstream target of pheromone signalling, however, led to a hypersporulation phenotype"
Keywords:		"Amino Acid Sequence Conserved Sequence Eremothecium/genetics/growth & development/*metabolism Fungal Proteins/genetics/*metabolism Gene Expression Regulation, Fungal/genetics Genes, Fungal/genetics Molecular Sequence Data Pheromones/genetics/*metabolism R;"
Notes:		"MedlineWendland, Jurgen Dunkler, Alexander Walther, Andrea eng Research Support, Non-U.S. Gov't England 2011/04/15 FEMS Yeast Res. 2011 Aug; 11(5):418-29. doi: 10.1111/j.1567-1364.2011.00732.x. Epub 2011 May 6"