| Title: | Identification of a new pheromone-binding protein in the antennae of a geometrid species and preparation of its antibody to analyze the antennal proteins of moths secreting type II sex pheromone components |
| Author(s): | Watanabe H; Tabunoki H; Miura N; Matsui A; Sato R; Ando T; |
| Address: | "Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan" |
| Journal Title: | Biosci Biotechnol Biochem |
| ISSN/ISBN: | 1347-6947 (Electronic) 0916-8451 (Linking) |
| Abstract: | "The full-length cDNA sequence of a new pheromone-binding protein (AscrPBP2) was determined from a geometrid moth, Ascotis selenaria cretacea, which secreted a Type II sex pheromone, and an antiserum against its recombinant protein overexpressed in Escherichia coli was prepared. In addition to this antiserum against AscrPBP2, antibodies against AscrPBP1 and general odorant-binding proteins of Bombyx mori were used in Western blotting experiments to analyze the proteins in the antennae of several lepidopteran species secreting Type II sex pheromone components" |
| Keywords: | "Amino Acid Sequence Animals Antibodies/immunology Base Sequence Bombyx DNA Primers DNA, Complementary Insect Proteins/chemistry/genetics/immunology/*metabolism Molecular Sequence Data Reverse Transcriptase Polymerase Chain Reaction Sex Attractants/*metabo;" |
| Notes: | "MedlineWatanabe, Hayaki Tabunoki, Hiroko Miura, Nami Matsui, Aya Sato, Ryoichi Ando, Tetsu eng England 2009/06/09 Biosci Biotechnol Biochem. 2009 Jun; 73(6):1443-6. doi: 10.1271/bbb.90029. Epub 2009 Jun 7" |
