| Title: | Differential impact of nutrition on developmental and metabolic gene expression during fruiting body development in Neurospora crassa |
| Author(s): | Wang Z; Lehr N; Trail F; Townsend JP; |
| Address: | "Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06520, USA" |
| DOI: | 10.1016/j.fgb.2012.03.004 |
| ISSN/ISBN: | 1096-0937 (Electronic) 1087-1845 (Print) 1087-1845 (Linking) |
| Abstract: | "Fungal fruiting body size and form are influenced by the ecology of the species, including diverse environmental stimuli. Accordingly, nutritional resources available to the fungus during development can be vital to successful production of fruiting bodies. To investigate the effect of nutrition, perithecial development of Neurospora crassa was induced on two different media, a chemically sparsely nutritive Synthetic Crossing Medium (SCM) and a natural Carrot Agar (CA). Protoperithecia were collected before crossing, and perithecia were collected at 2, 24, 48, 72, 96, 120, and at full maturity 144 h after crossing. No differences in fruiting body morphology were observed between the two media at any time point. A circuit of microarray hybridizations comparing cDNA from all neighboring stages was performed. For a majority of differentially expressed genes, expression was higher in SCM than in CA, and expression of core metabolic genes was particularly affected. Effects of nutrition were highest in magnitude before crossing, lowering in magnitude during early perithecial development. Interestingly, metabolic effects of the media were also large in magnitude during late perithecial development, at which stage the lower expression in CA presumably reflected the continued intake of diverse complex initial compounds, diminishing the need for expression of anabolic pathways. However, for genes with key regulatory roles in sexual development, including pheromone precursor ccg-4 and poi2, expression patterns were similar between treatments. When possible, a common nutritional environment is ideal for comparing transcriptional profiles between different fungi. Nevertheless, the observed consistency of the developmental program across media, despite considerable metabolic differentiation is reassuring. This result facilitates comparative studies that will require different nutritional resources for sexual development in different fungi" |
| Keywords: | "Culture Media/chemistry DNA, Complementary/genetics DNA, Fungal/genetics Fruiting Bodies, Fungal/cytology/*genetics/*growth & development Gene Expression Profiling *Gene Expression Regulation, Fungal Metabolic Networks and Pathways/genetics Microarray Ana;" |
| Notes: | "MedlineWang, Zheng Lehr, Nina Trail, Frances Townsend, Jeffrey P eng P01 GM068087/GM/NIGMS NIH HHS/ P01 GM068067/GM/NIGMS NIH HHS/ Comparative Study Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. 2012/04/04 Fungal Genet Biol. 2012 May; 49(5):405-13. doi: 10.1016/j.fgb.2012.03.004. Epub 2012 Mar 26" |
