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Int J Food Microbiol

Title:		"2,4-Di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated Lactococcus sp"
Author(s):		Varsha KK; Devendra L; Shilpa G; Priya S; Pandey A; Nampoothiri KM;
Address:		"Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India. Agroprocessing Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India. Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum 695 019, Kerala, India. Electronic address: madhavan85@hotmail.com"
Journal Title:		Int J Food Microbiol
Year:		2015
Volume:		20150702
Issue:
Page Number:		44 - 50
DOI:		10.1016/j.ijfoodmicro.2015.06.025
ISSN/ISBN:		1879-3460 (Electronic) 0168-1605 (Linking)
Abstract:		"The volatile organic compound 2,4-di-tert-butyl phenol (2,4 DTBP) was purified from the cell free supernatant of a newly isolated Lactococcus sp. by solvent extraction and chromatographic techniques. Molecular characterization of the compound by ESI-MS, (1)H NMR and FTIR analysis revealed the structure, C14H22O. Fungicidal activity was demonstrated against Aspergillus niger, Fusarium oxysporum and Penicillium chrysogenum by disc diffusion assay. Among the cell lines tested for cytotoxicity of this compound (normal cell line H9c2 and cancer cell lines HeLa and MCF-7), a remarkable cytotoxicity against HeLa cells with an IC50 value of 10 mug/mL was shown. A biocontrol experiment with 2,4 DTBP supplemented fraction prevented growth of the abovementioned fungi on wheat grains. The study further strengthens the case for development of biopreservatives and dietary antioxidants from lactic acid bacteria for food applications"
Keywords:		"Antifungal Agents/chemistry/*isolation & purification/*pharmacology Antioxidants/chemistry/*isolation & purification/*pharmacology Aspergillus niger/drug effects/growth & development Cell Line, Tumor Cell Survival Fusarium/drug effects/growth & developmen;"
Notes:		"MedlineVarsha, Kontham Kulangara Devendra, Leena Shilpa, Ganesan Priya, Sulochana Pandey, Ashok Nampoothiri, Kesavan Madhavan eng Research Support, Non-U.S. Gov't Netherlands 2015/07/15 Int J Food Microbiol. 2015 Oct 15; 211:44-50. doi: 10.1016/j.ijfoodmicro.2015.06.025. Epub 2015 Jul 2"