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FEBS Lett

Title:		Glucosylation of chimeric proteins in the cell wall of Saccharomyces cerevisiae
Author(s):		van Berkel MA; Caro LH; Montijn RC; Klis FM;
Address:		"Institute for Molecular Cell Biology, University of Amsterdam, BioCentrum Amsterdam, The Netherlands"
Journal Title:		FEBS Lett
Year:		1994
Volume:		349
Issue:		1
Page Number:		135 - 138
DOI:		10.1016/0014-5793(94)00631-8
ISSN/ISBN:		0014-5793 (Print) 0014-5793 (Linking)
Abstract:		"Extension of a reporter protein with the carboxyterminal thirty amino acids of the cell wall mannoprotein alpha-agglutinin of Saccharomyces cerevisiae resulted in incorporation of the chimeric protein in the cell wall. By Western analysis it was shown that the incorporated protein contained beta-1,6-glucan similar to endogenous cell wall proteins, whereas excreted reporter protein was not glucosylated. This suggests that beta-1,6-glucan is involved in anchoring mannoproteins in the cell wall"
Keywords:		"Cell Wall/*metabolism Genes, Reporter Glucans/*metabolism Glucose/metabolism Glycoproteins/*metabolism Glycosylation Mating Factor Membrane Glycoproteins/metabolism Peptides/genetics/*metabolism Saccharomyces cerevisiae/genetics/*metabolism *beta-Glucans;"
Notes:		"Medlinevan Berkel, M A Caro, L H Montijn, R C Klis, F M eng England 1994/07/25 FEBS Lett. 1994 Jul 25; 349(1):135-8. doi: 10.1016/0014-5793(94)00631-8"