| Title: | Neomycin inhibition of (+)-7-iso-jasmonoyl-L-isoleucine accumulation and signaling |
| Author(s): | Vadassery J; Reichelt M; Jimenez-Aleman GH; Boland W; Mithofer A; |
| Address: | "Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knoll-Str. 8, 07745, Jena, Germany, jvadassery@ice.mpg.de" |
| DOI: | 10.1007/s10886-014-0448-7 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "The majority of plant defenses against insect herbivores are coordinated by jasmonate (jasmonic acid, JA; (+)-7-iso-jasmonoyl-L-isoleucine, JA-Ile)-dependent signaling cascades. Insect feeding and mimicking herbivory by application of oral secretions (OS) from the insect induced both cytosolic Ca(2+) and jasmonate-phytohormone elevation in plants. Here it is shown that in Arabidopsis thaliana upon treatment with OS from lepidopteran Spodoptera littoralis larvae, the antibiotic neomycin selectively blocked the accumulation of OS-induced Ca(2+) elevation and level of the bioactive JA-Ile, in contrast to JA level. Furthermore, neomycin treatment affected the downstream expression of JA-Ile-responsive genes, VSP2 and LOX2, in Arabidopsis. The neomycin-dependent reduced JA-Ile level is partially due to increased CYP94B3 expression and subsequent JA-Ile turn-over to12-hydroxy-JA-Ile. It is neither due to the inhibition of the enzymatic conjugation process nor to substrate availability. Thus, blocking Ca(2+) elevation specifically controls JA-Ile accumulation and signaling, offering an insight into role of calcium in defense against insect herbivory" |
| Keywords: | "Animals Anti-Bacterial Agents/pharmacology Arabidopsis/*chemistry/metabolism Arabidopsis Proteins/genetics/metabolism Calcium/metabolism Cyclopentanes/chemistry/*metabolism Cytochrome P-450 Enzyme System/metabolism Gene Expression Regulation, Plant/drug e;" |
| Notes: | "MedlineVadassery, Jyothilakshmi Reichelt, Michael Jimenez-Aleman, Guillermo H Boland, Wilhelm Mithofer, Axel eng 2014/05/27 J Chem Ecol. 2014 Jul; 40(7):676-86. doi: 10.1007/s10886-014-0448-7. Epub 2014 May 25" |
