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J Breath Res

Title:		Exhaled breath and oral cavity VOCs as potential biomarkers in oral cancer patients
Author(s):		Bouza M; Gonzalez-Soto J; Pereiro R; de Vicente JC; Sanz-Medel A;
Address:		"Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, E-33006 Oviedo, Spain"
Journal Title:		J Breath Res
Year:		2017
Volume:		20170301
Issue:		1
Page Number:		16015 -
DOI:		10.1088/1752-7163/aa5e76
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Linking)
Abstract:		"Corporal mechanisms attributed to cancer, such as oxidative stress or the action of cytochrome P450 enzymes, seem to be responsible for the generation of a variety of volatile organic compounds (VOCs) that could be used as non-invasive diagnosis biomarkers. The present work presents an attempt to use VOCs from exhaled breath and oral cavity air as biomarkers for oral squamous cell carcinoma (OSCC) patients. A total of 52 breath samples were collected (in 3 L Tedlar bags) from 26 OSCC patients and 26 cancer-free controls. The samples were analyzed using solid-phase microextraction followed by gas chromatography-mass spectrometry detection. Different statistical strategies (e.g., Icoshift, SIMCA, LDA, etc) were used to classify the analytical data. Results revealed that compounds such as undecane, dodecane, decanal, benzaldehyde, 3,7-dimethyl undecane, 4,5-dimethyl nonane, 1-octene, and hexadecane had relevance as possible biomarkers for OSCC. LDA classification with these compounds showed well-defined clusters for patients and controls (non-smokers and smokers). In addition to breath analysis, preliminary studies were carried out to evaluate the possibility of lesion-surrounded air (analyzed OSCC tumors are in the oral cavity) as a source of biomarkers. The oral cavity location of the squamous cell carcinoma tumors constitutes an opportunity to non-invasively collect the air surrounding the lesion. Small quantities (20 ml) of air collected in the oral cavity were analyzed using the above methodology. Results showed that aldehydes present in the oral cavity might constitute potential OSCC biomarkers"
Keywords:		"Adult Aged Air Biomarkers/analysis Breath Tests/*methods Carcinoma, Squamous Cell/diagnosis/metabolism/pathology Discriminant Analysis *Exhalation Female Humans Male Middle Aged Mouth/chemistry Mouth Neoplasms/*diagnosis/*metabolism/pathology Volatile Org;"
Notes:		"MedlineBouza, M Gonzalez-Soto, J Pereiro, R de Vicente, J C Sanz-Medel, A eng England 2017/02/07 J Breath Res. 2017 Mar 1; 11(1):016015. doi: 10.1088/1752-7163/aa5e76"