| Title: | Elevated levels of proinflammatory volatile metabolites in feces of high fat diet fed KK-A(y) mice |
| Author(s): | Uchikawa M; Kato M; Nagata A; Sanada S; Yoshikawa Y; Tsunematsu Y; Sato M; Suzuki T; Hashidume T; Watanabe K; Yoshikawa Y; Miyoshi N; |
| Address: | "School of Food Nutritional Sciences, Shizuoka, Japan. Graduate School of Integrated Pharmaceutical and Nutritional Sciences, Shizuoka, Japan. School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan. Food Environmental Design Course, Faculty of Education, Art and Science, Yamagata University, Yamagata, Japan. School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan. School of Food Nutritional Sciences, Shizuoka, Japan. miyoshin@u-shizuoka-ken.ac.jp. School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan. miyoshin@u-shizuoka-ken.ac.jp" |
| DOI: | 10.1038/s41598-020-62541-7 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "When the microfloral composition deteriorates, it triggers low-level chronic inflammation associated with several lifestyle-related diseases including obesity and diabetic mellitus. Fecal volatile organic compounds (VOCs) have been found to differ in gastrointestinal diseases as well as intestinal infection. In this study, to evaluate a potential association between the pathogenesis of lifestyle-related diseases and VOCs in the intestinal tract, fecal VOCs from obese/diabetic KK-A(y) mice (KK) or controls (C57BL/6J mice; BL) fed a normal or high fat diet (NFD or HFD) were investigated using headspace sampler-GC-EI-MS. Principal component analysis (PCA) of fecal VOC profiles clearly separated the experimental groups depending on the mouse lineage (KK vs BL) and the diet type (NFD vs HFD). 16 s rRNA sequencing revealed that the PCA distribution of VOCs was in parallel with the microfloral composition. We identified that some volatile metabolites including n-alkanals (nonanal and octanal), acetone and phenol were significantly increased in the HFD and/or KK groups. Additionally, these volatile metabolites induced proinflammatory activity in the RAW264 murine macrophage cell line indicating these bioactive metabolites might trigger low-level chronic inflammation. These results suggest that proinflammatory VOCs detected in HFD-fed and/or diabetic model mice might be novel noninvasive diagnosis biomarkers for diabetes" |
| Keywords: | "Animals Biomarkers/analysis Diet, High-Fat/*adverse effects Disease Models, Animal Feces/chemistry Gastrointestinal Microbiome/genetics Inflammation Male Mice Mice, Inbred C57BL Mice, Obese Obesity/*metabolism RAW 264.7 Cells RNA, Ribosomal, 16S/genetics;" |
| Notes: | "MedlineUchikawa, Misaki Kato, Mai Nagata, Akika Sanada, Shunsuke Yoshikawa, Yuto Tsunematsu, Yuta Sato, Michio Suzuki, Takuji Hashidume, Tsutomu Watanabe, Kenji Yoshikawa, Yuko Miyoshi, Noriyuki eng Research Support, Non-U.S. Gov't England 2020/04/02 Sci Rep. 2020 Mar 30; 10(1):5681. doi: 10.1038/s41598-020-62541-7" |
