| Title: | Geranyl modification on the tryptophan residue of ComXRO-E-2 pheromone by a cell-free system |
| Author(s): | Tsuji F; Ishihara A; Kurata K; Nakagawa A; Okada M; Kitamura S; Kanamaru K; Masuda Y; Murakami K; Irie K; Sakagami Y; |
| Address: | "Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, Aichi 464-8601, Japan" |
| DOI: | 10.1016/j.febslet.2011.12.012 |
| ISSN/ISBN: | 1873-3468 (Electronic) 0014-5793 (Linking) |
| Abstract: | "ComX pheromone is an isoprenoidal oligopeptide containing a modified tryptophan residue, which stimulates natural genetic competence in the gram-positive bacterium Bacillus. Since posttranslational prenylation on the tryptophan residue has not been reported except in ComX pheromone, the universality of this modification has not yet been elucidated. In this paper, we established a cell-free system, whereby the tryptophan residue in peptides is modified with a geranyl group by modifying enzyme ComQ. In addition, we investigated enzymatic reaction conditions using an in vitro enzyme reaction system. This is the first report of in vitro geranylation on the tryptophan residue. This system is potentially a useful tool for elucidating the universality of prenylation on the tryptophan residue" |
| Keywords: | "Amino Acid Sequence Bacillus subtilis/metabolism Bacterial Proteins/chemistry/*metabolism Catalysis Cell-Free System Geranyltranstransferase/*metabolism In Vitro Techniques Models, Biological Molecular Sequence Data Pheromones/chemistry/metabolism *Protei;" |
| Notes: | "MedlineTsuji, Fumitada Ishihara, Ayako Kurata, Keiko Nakagawa, Aya Okada, Masahiro Kitamura, Shigeyuki Kanamaru, Kyoko Masuda, Yuichi Murakami, Kazuma Irie, Kazuhiro Sakagami, Youji eng Research Support, Non-U.S. Gov't England 2011/12/27 FEBS Lett. 2012 Jan 20; 586(2):174-9. doi: 10.1016/j.febslet.2011.12.012. Epub 2011 Dec 17" |
