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Cell Cycle

Title:		Co-regulation of polar mRNA transport and lifespan in budding yeast Saccharomyces cerevisiae
Author(s):		Taranukha D; Budovsky A; Gobshtis N; Braiman A; Porat Z; Aronov S; Fraifeld VE;
Address:		"The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, Beer-Sheva, Israel"
Journal Title:		Cell Cycle
Year:		2012
Volume:		20121030
Issue:		22
Page Number:		4275 - 4280
DOI:		10.4161/cc.22659
ISSN/ISBN:		1551-4005 (Electronic) 1538-4101 (Print) 1551-4005 (Linking)
Abstract:		"Recent studies have uncovered the links between aging, rejuvenation and polar protein transport in the budding yeast Saccharomyces cerevisiae. Here, we examined a still unexplored possibility for co-regulation of polar mRNA transport and lifespan. To monitor the amount and distribution of mRNA-containing granules in mother and daughter cells, we used a fluorescent mRNA-labeling system, with MFA2 as a reporter gene. The results obtained showed that deletion of the selected longevity regulators in budding yeast had a significant impact on the polar mRNA transport. This included changes in the amount of mRNA-containing granules in cytoplasm, their aggregation and distribution between the mother and daughter cells. A significant negative correlation was found between strain-specific longevity, amount of granules and total fluorescent intensity both in mother and daughter cells. As indicated by the coefficient of determination, approximately 50-75% of variation in yeast lifespan could be attributed to the differences in polar mRNA transport"
Keywords:		"Fluorescent Dyes/chemistry Genes, Reporter Lipoproteins/genetics/metabolism *Longevity Microscopy, Confocal Pheromones/genetics/metabolism RNA Transport RNA, Messenger/*metabolism Saccharomyces cerevisiae/*metabolism Saccharomyces cerevisiae Proteins/gene;"
Notes:		"MedlineTaranukha, Dmitri Budovsky, Arie Gobshtis, Nikolai Braiman, Alex Porat, Ziv Aronov, Stella Fraifeld, Vadim E eng Research Support, Non-U.S. Gov't 2012/11/01 Cell Cycle. 2012 Nov 15; 11(22):4275-80. doi: 10.4161/cc.22659. Epub 2012 Oct 30"