| Title: | A new combined green method for 2-Chlorophenol removal using cross-linked Brassica rapa peroxidase in silicone oil |
| Author(s): | Tandjaoui N; Abouseoud M; Couvert A; Amrane A; Tassist A; |
| Address: | "Laboratoire de Biomateriaux et Phenomenes de Transport, Faculte des Sciences et de la Technologie, Universite Yahia Fares de Medea, Pole Universitaire, RN1, Medea 26000 Algeria; Ecole Nationale Superieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allee de Beaulieu, CS 50837, 35708 Rennes Cedex 7, France. Electronic address: nassima_2900@yahoo.fr. Laboratoire de Biomateriaux et Phenomenes de Transport, Faculte des Sciences et de la Technologie, Universite Yahia Fares de Medea, Pole Universitaire, RN1, Medea 26000 Algeria; Laboratoire de Genie de la Reaction, Faculte de Genie Mecanique et Genie des Procedes, Universite Houari Boumediene, Bab Ezzouar, Alger 16111, Algeria. Ecole Nationale Superieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allee de Beaulieu, CS 50837, 35708 Rennes Cedex 7, France; Universite europeenne de Bretagne, 5 Boulevard Laennec, 35000 Rennes, France. Laboratoire de Genie de la Reaction, Faculte de Genie Mecanique et Genie des Procedes, Universite Houari Boumediene, Bab Ezzouar, Alger 16111, Algeria" |
| DOI: | 10.1016/j.chemosphere.2016.01.021 |
| ISSN/ISBN: | 1879-1298 (Electronic) 0045-6535 (Linking) |
| Abstract: | "This study proposes a new technique to treat waste air containing 2-Chlorophenol (2-CP), namely an integrated process coupling absorption of the compound in an organic liquid phase and its enzymatic degradation. Silicone oil (47V20) was used as an organic absorbent to allow the volatile organic compound (VOC) transfer from the gas phase to the liquid phase followed by its degradation by means of Cross-linked Brassica rapa peroxidase (BRP) contained in the organic phase. An evaluation of silicone oil (47V20) absorption capacity towards 2-CP was first accomplished by determining its partition coefficient (H) in this solvent. The air-oil partition coefficient of 2-CP was found equal to 0.136 Pa m(3) mol(-1), which is five times lower than the air-water value (0.619 Pam(3) mol(-1)). The absorbed 2-CP was then subject to enzymatic degradation by cross-linked BRP aggregates (BRP-CLEAs). The degradation step was affected by four parameters (contact time; 2-CP, hydrogen peroxide and enzyme concentrations), which were optimized in order to obtain the highest conversion yield. A maximal conversion yield of 69% and a rate of 1.58 mg L(-1) min(-1)were obtained for 100 min duration time when 2-CP and hydrogen peroxide concentrations were respectively 80 mg L(-1) and 6 mM in the presence of 2.66 UI mL(-1) BRP-CLEAs. The reusability of BRP-CLEAs in silicone oil was assessed, showing promising results since 59% of their initial efficiency remained after three batches" |
| Keywords: | Adsorption Air Pollutants/*isolation & purification Brassica rapa/*enzymology Catalysis Chlorophenols/*isolation & purification Cross-Linking Reagents/chemistry Glutaral/chemistry Hydrogen Peroxide/chemistry Peroxidase/*chemistry Silicone Oils/*chemistry; |
| Notes: | "MedlineTandjaoui, Nassima Abouseoud, Mahmoud Couvert, Annabelle Amrane, Abdeltif Tassist, Amina eng England 2016/01/24 Chemosphere. 2016 Apr; 148:55-60. doi: 10.1016/j.chemosphere.2016.01.021. Epub 2016 Jan 21" |
