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PLoS One

Title:		Identification of characteristic compounds of moderate volatility in breast cancer cell lines
Author(s):		Tanaka M; Hsuan C; Oeki M; Shen W; Goda A; Tahara Y; Onodera T; Sanematsu K; Rikitake T; Oki E; Ninomiya Y; Kurebayashi R; Sonoda H; Maehara Y; Toko K; Matsui T;
Address:		"Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan. Research and Development Center for Five-Sense Devices, Kyushu University, Fukuoka, Japan. Faculty of Information Science and Electrical Engineering, Kyushu University, Fukuoka, Japan. Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan. Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. JOHNAN Co., Kyoto, Japan. Department of General Surgery, Imari-Arita Kyoritsu Hospital, Saga, Japan. Institute for Advanced Study, Kyushu University, Fukuoka, Japan"
Journal Title:		PLoS One
Year:		2020
Volume:		20200629
Issue:		6
Page Number:		e0235442 -
DOI:		10.1371/journal.pone.0235442
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"In this study, we were challenging to identify characteristic compounds in breast cancer cell lines. GC analysis of extracts from the culture media of breast cancer cell lines (MCF-7, SK-BR-3, and YMB-1) using a solid-phase Porapak Q extraction revealed that two compounds of moderate volatility, 1-hexadecanol and 5-(Z)-dodecenoic acid, were detected with markedly higher amount than those in the medium of fibroblast cell line (KMST-6). Furthermore, LC-TOF/MS analysis of the extracts clarified that in addition to the above two fatty acids, the amounts of five unsaturated fatty acids [decenoic acid (C10:1), decadienoic acid (C10:2), 5-(Z)-dodecenoic acid (C12:1), 5-(Z)-tetradecenoic acid (C14:1), and tetradecadienoic acid (C14:2)] in MCF-7 medium were higher than those in medium of KMST-6. Interestingly, H2O2-oxidation of 5-(Z)-dodecenoic acid and 5-(Z)-tetradecenoic acid produced volatile aldehydes that were reported as specific volatiles in breath from various cancer patients, such as heptanal, octanal, nonanal, decanal, 2-(E)-nonenal, and 2-(E)-octenal. Thus, we concluded that these identified compounds over-produced in breast cancer cells in this study could serve as potential precursors producing reported cancer-specific volatiles"
Keywords:		"Breast Neoplasms/*metabolism/*pathology Fatty Acids/analysis/*metabolism Female Gas Chromatography-Mass Spectrometry Humans Oxidation-Reduction Solid Phase Microextraction Tumor Cells, Cultured Volatile Organic Compounds/analysis/*metabolism;neuroscience;"
Notes:		"MedlineTanaka, Mitsuru Hsuan, Chung Oeki, Masataka Shen, Weilin Goda, Asuka Tahara, Yusuke Onodera, Takeshi Sanematsu, Keisuke Rikitake, Tomotsugu Oki, Eiji Ninomiya, Yuzo Kurebayashi, Rintaro Sonoda, Hideto Maehara, Yoshihiko Toko, Kiyoshi Matsui, Toshiro eng Research Support, Non-U.S. Gov't 2020/07/01 PLoS One. 2020 Jun 29; 15(6):e0235442. doi: 10.1371/journal.pone.0235442. eCollection 2020"