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Front Microbiol

Title:		Improving the Activity of Antimicrobial Peptides Against Aquatic Pathogen Bacteria by Amino Acid Substitutions and Changing the Ratio of Hydrophobic Residues
Author(s):		Tan R; Wang M; Xu H; Qin L; Wang J; Cui P; Ru S;
Address:		"College of Marine Life Science, Ocean University of China, Qingdao, China"
Journal Title:		Front Microbiol
Year:		2021
Volume:		20211018
Issue:
Page Number:		773076 -
DOI:		10.3389/fmicb.2021.773076
ISSN/ISBN:		1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking)
Abstract:		"With the increasing number of drug-resistant bacteria, there is an urgent need for new antimicrobial agents, and antimicrobial peptides (AMPs), which exist in the human non-specific immune system, are one of the most promising candidates. It is an effective optimization strategy to modify antimicrobial peptides (AMPs) according to the distribution of amino acids and hydrophobic characteristics. The addition of bacterial pheromones to the N short peptide can increase the ability to recognize bacteria. In this study, we designed and synthesized AMP1-6 by amino acid substitution of mBjAMP1. Additionally, P-6, S-6, and L-6 were designed and synthesized by adding bacterial pheromones based on 1-6. Functional tests showed that the four AMPs had the ability to kill Gram-negative Vibrio anguillarum, Pseudomonas mendocina, and Vibrio parahaemolyticus, and Gram-positive Micrococcus luteus and Listeria monocytogenes. Additionally, all four AMPs induced permeabilization and depolarization of bacterial cell membranes and increased intracellular reactive oxygen species (ROS) levels. Importantly, they had little or no mammalian cytotoxicity. At the same time, 1-6 and L-6 protected the stability of intestinal flora in Sebastes schlegelii and increased the relative abundance of Lactobacillaceae. In summary, our results indicate that the designed AMPs have broad application prospects as a new type of polypeptide antimicrobial agent"
Keywords:		amino acid substitutions antibiotics antimicrobial peptides (AMP) aquatic pathogen bacteria structure-based design;
Notes:		"PubMed-not-MEDLINETan, Rong Wang, Meiru Xu, Huiqin Qin, Lu Wang, Jun Cui, Pengfei Ru, Shaoguo eng Switzerland 2021/11/05 Front Microbiol. 2021 Oct 18; 12:773076. doi: 10.3389/fmicb.2021.773076. eCollection 2021"