| Title: | High-energy-level metabolism and transport occur at the transition from closed to open flowers |
| Author(s): | Borghi M; Perez de Souza L; Tohge T; Mi J; Melandri G; Proost S; Martins MCM; Al-Babili S; Bouwmeester HJ; Fernie AR; |
| Address: | "Department of Biology, Utah State University, Logan, Utah 84321-5305, USA. Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany. Laboratory of Plant Physiology, Wageningen University and Research, Wageningen 6708 PB, The Netherlands. Nara Institute of Science and Technology, Nara 630-0192, Japan. The Bioactives Lab, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia. INRAE, University of Bordeaux, UMR BFP, Villenave d'Ornon 33140, France. Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, Belgium. Center for Microbiology, VIB, Leuven 3000, Belgium. In Press-Consultoria e Comunicacao Cientifica, Sao Paulo 05089-030, Brazil. Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands" |
| ISSN/ISBN: | 1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking) |
| Abstract: | "During the maturation phase of flower development, the onset of anthesis visibly marks the transition from buds to open flowers, during which petals stretch out, nectar secretion commences, and pollination occurs. Analysis of the metabolic changes occurring during this developmental transition has primarily focused on specific classes of metabolites, such as pigments and scent emission, and far less on the whole network of primary and secondary metabolites. To investigate the metabolic changes occurring at anthesis, we performed multi-platform metabolomics alongside RNA sequencing in individual florets harvested from the main inflorescence of Arabidopsis (Arabidopsis thaliana) ecotype Col-0. To trace metabolic fluxes at the level of the whole inflorescence and individual florets, we further integrated these studies with radiolabeled experiments. These extensive analyses revealed high-energy-level metabolism and transport of carbohydrates and amino acids, supporting intense metabolic rearrangements occurring at the time of this floral transition. These comprehensive data are discussed in the context of our current understanding of the metabolic shifts underlying flower opening. We envision that this analysis will facilitate the introgression of floral metabolic traits promoting pollination in crop species for which a comprehensive knowledge of flower metabolism is still limited" |
| Keywords: | *Flowers Inflorescence Odorants *Pollination Reproduction; |
| Notes: | "MedlineBorghi, Monica Perez de Souza, Leonardo Tohge, Takayuki Mi, Jianing Melandri, Giovanni Proost, Sebastian Martins, Marina C M Al-Babili, Salim Bouwmeester, Harro J Fernie, Alisdair R eng Research Support, Non-U.S. Gov't 2022/06/01 Plant Physiol. 2022 Aug 29; 190(1):319-339. doi: 10.1093/plphys/kiac253" |
