| Title: | "Application of liquid chromatography-nuclear magnetic resonance spectroscopy for the identification of ethyldimethylpyrazine, a food flavouring agent" |
| Author(s): | Sugimoto N; Yomota C; Furusho N; Sato K; Yamazaki T; Tanamoto K; |
| Address: | "nsNational Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo 158-8501, Japan. nsugimot@nihs.go.jp" |
| DOI: | 10.1080/02652030600871168 |
| ISSN/ISBN: | 0265-203X (Print) 0265-203X (Linking) |
| Abstract: | "The application of liquid chromatography-nuclear magnetic resonance spectroscopy (LC-NMR) for the direct identification of ethyldimethylpyrazine, a food flavouring agent, has been studied. The commercial product is a mixture of two regio-isomers, 2-ethyl-3,5-dimethylpyrazine (1) and 2-ethyl-3,6-dimethylpyrazine (2); however, the exact composition of the mixture is unknown. Structural characterization by LC-MS and GC-MS was not possible because both regio-isomers yield the same molecular related ion and ion fragmentation. To rapidly identify the two regio-isomers, the product was analyzed by LC-NMR with on-flow and fraction loop modes. From the results, the structure elucidations of the two regio-isomers could be carried out without the need to isolate the isomers by the usual procedures" |
| Keywords: | "Chromatography, Liquid/methods Flavoring Agents/*analysis Food Additives/*analysis Food Analysis/methods Gas Chromatography-Mass Spectrometry/methods Magnetic Resonance Spectroscopy/methods Pheromones/analysis Pyrazines/*analysis;" |
| Notes: | "MedlineSugimoto, Naoki Yomota, Chikako Furusho, Noriko Sato, Kyoko Yamazaki, Takeshi Tanamoto, Kenichi eng Research Support, Non-U.S. Gov't England 2006/11/23 Food Addit Contam. 2006 Dec; 23(12):1253-9. doi: 10.1080/02652030600871168" |
