| Title: | Construction of vectors for inducible gene expression in Lactobacillus sakei and L plantarum |
| Author(s): | Sorvig E; Gronqvist S; Naterstad K; Mathiesen G; Eijsink VG; Axelsson L; |
| Address: | "Matforsk, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway. elisabeth.sorvig@matforsk.no" |
| DOI: | 10.1016/S0378-1097(03)00798-5 |
| ISSN/ISBN: | 0378-1097 (Print) 0378-1097 (Linking) |
| Abstract: | "We have constructed vectors for inducible expression of genes in Lactobacillus sakei and Lactobacillus plantarum. The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and sakacin P and the genes encoding the cognate histidine protein kinase and response regulator that are necessary to activate this promoter upon induction by a peptide pheromone. The vectors are built up of cassettes that permit easy exchange of all parts through restriction enzyme digestion and ligation. Using beta-glucuronidase as a reporter enzyme, variants of these vectors were compared with each other, and with a corresponding system based on genes involved in the production of nisin. Several of the new vectors permitted tightly controlled and efficient expression of beta-glucuronidase in both L. sakei and L. plantarum" |
| Keywords: | "Gene Expression Regulation, Bacterial Genes, Reporter *Genetic Vectors Glucuronidase/genetics Lactobacillus/*genetics *Plasmids;" |
| Notes: | "MedlineSorvig, Elisabeth Gronqvist, Sonja Naterstad, Kristine Mathiesen, Geir Eijsink, Vincent G H Axelsson, Lars eng Research Support, Non-U.S. Gov't England 2003/12/09 FEMS Microbiol Lett. 2003 Dec 5; 229(1):119-26. doi: 10.1016/S0378-1097(03)00798-5" |
