| Title: | Exhaled volatilome analysis as a useful tool to discriminate asthma with other coexisting atopic diseases in women of childbearing age |
| Author(s): | Sola-Martinez RA; Lozano-Terol G; Gallego-Jara J; Morales E; Cantero-Cano E; Sanchez-Solis M; Garcia-Marcos L; Jimenez-Guerrero P; Noguera-Velasco JA; Canovas Diaz M; de Diego Puente T; group Ns; |
| Address: | "Department of Biochemistry and Molecular Biology B and Immunology, University of Murcia, Murcia, Spain. Biomedical Research Institute of Murcia, IMIB-Arrixaca, Murcia, Spain. Department of Public Health Sciences, University of Murcia, Murcia, Spain. Respiratory and Allergy Units, Arrixaca Children's University Hospital, University of Murcia, Murcia, Spain. Department of Paediatrics, University of Murcia, Murcia, Spain. Network of Asthma and Adverse and Allergy Reactions (ARADyAL), Health Institute Carlos III, Madrid, Spain. Regional Atmospheric Modelling Group, Department of Physics, University of Murcia, Murcia, Spain. Molecular Therapy and Biomarkers Research Group, Clinical Analysis Service, University Clinical Hospital 'Virgen de la Arrixaca', University of Murcia, Murcia, Spain. Department of Biochemistry and Molecular Biology B and Immunology, University of Murcia, Murcia, Spain. tdp@um.es. Biomedical Research Institute of Murcia, IMIB-Arrixaca, Murcia, Spain. tdp@um.es" |
| DOI: | 10.1038/s41598-021-92933-2 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "The prevalence of asthma is considerably high among women of childbearing age. Most asthmatic women also often have other atopic disorders. Therefore, the differentiation between patients with atopic diseases without asthma and asthmatics with coexisting diseases is essential to avoid underdiagnosis of asthma and to design strategies to reduce symptom severity and improve quality of life of patients. Hence, we aimed for the first time to conduct an analysis of volatile organic compounds in exhaled breath of women of childbearing age as a new approach to discriminate between asthmatics with other coexisting atopic diseases and non-asthmatics (with or without atopic diseases), which could be a helpful tool for more accurate asthma detection and monitoring using a noninvasive technique in the near future. In this study, exhaled air samples of 336 women (training set (n = 211) and validation set (n = 125)) were collected and analyzed by thermal desorption coupled with gas chromatography-mass spectrometry. ASCA (ANOVA (analysis of variance) simultaneous component analysis) and LASSO + LS (least absolute shrinkage and selection operator + logistic regression) were employed for data analysis. Fifteen statistically significant models (p-value < 0.05 in permutation tests) that discriminated asthma with other coexisting atopic diseases in women of childbearing age were generated. Acetone, 2-ethyl-1-hexanol and a tetrahydroisoquinoline derivative were selected as discriminants of asthma with other coexisting atopic diseases. In addition, carbon disulfide, a tetrahydroisoquinoline derivative, 2-ethyl-1-hexanol and decane discriminated asthma disease among patients with other atopic disorders. Results of this study indicate that refined metabolomic analysis of exhaled breath allows asthma with other coexisting atopic diseases discrimination in women of reproductive age" |
| Keywords: | "Adult Asthma/*diagnosis/metabolism/pathology Breath Tests Diagnosis, Differential *Exhalation Female Gas Chromatography-Mass Spectrometry Humans Hypersensitivity, Immediate/*diagnosis/metabolism/pathology Male Quality of Life Volatile Organic Compounds/*i;" |
| Notes: | "MedlineSola-Martinez, Rosa A Lozano-Terol, Gema Gallego-Jara, Julia Morales, Eva Cantero-Cano, Esther Sanchez-Solis, Manuel Garcia-Marcos, Luis Jimenez-Guerrero, Pedro Noguera-Velasco, Jose A Canovas Diaz, Manuel de Diego Puente, Teresa eng Research Support, Non-U.S. Gov't England 2021/07/07 Sci Rep. 2021 Jul 5; 11(1):13823. doi: 10.1038/s41598-021-92933-2" |
