| Title: | Evolution of sex pheromone receptors in Dendrolimus punctatus Walker (lepidoptera: Lasiocampidae) is divergent from other moth species |
| Author(s): | Shen S; Cao S; Zhang Z; Kong X; Liu F; Wang G; Zhang S; |
| Address: | "Key Laboratory of Forest Protection of State Forestry and Grassland Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, 100091, Beijing, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China. Electronic address: wangguirong@caas.cn. Key Laboratory of Forest Protection of State Forestry and Grassland Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, 100091, Beijing, China. Electronic address: zhangsf@caf.ac.cn" |
| DOI: | 10.1016/j.ibmb.2020.103375 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "Dendrolimus punctatus Walker (Lepidoptera: Lasiocampidae) is a pine caterpillar moth distributed in most areas of southern China and is an economically important pest of pine, due to its defoliation activity. Understanding fundamental sex pheromone perception mechanisms in D. punctatus may provide effective and sustainable options for novel control strategies. However, the identification and function of pheromone receptors, key genes that receipt the pheromone of this pest, are both unclear now. Previous researches suggested several candidate pheromone receptors whose expression levels were male antennae bias in D. punctatus. In this study, we cloned six candidate pheromone receptors (DpunOR 20/45/46/51/54/58) and Orco from D. punctatus. Phylogenetic tree analysis showed that lepidopteran PRs tend to be conserved and clustered together; however, D. punctatus candidate PRs were located in a distinct clade. Motif analysis of PRs showed clear sequences differences between Dendrolimus spp. and other tested moth species. To illustrate the ligand response properties of the candidate PRs of D. punctatus, each of the six genes was expressed with an Orco gene in Xenopus oocytes and using two-electrode voltage-clamp recordings. Finally, we successfully identified two sex pheromone receptors (PR45 and PR46). Our study, which identified a novel lineage of PRs tuned to Type I pheromones in Lepidoptera, provides evidence for the new evolution origin of sex pheromone communication in moths, and lays a foundation for the development of novel control strategies of D. punctatus" |
| Keywords: | "Amino Acid Sequence Animals *Evolution, Molecular Insect Proteins/chemistry/*genetics/metabolism Male Moths/*genetics/metabolism Phylogeny Receptors, Pheromone/chemistry/*genetics/metabolism Sequence Alignment Sex Attractants/*metabolism Evolution Functio;" |
| Notes: | "MedlineShen, Sifan Cao, Song Zhang, Zhen Kong, Xiangbo Liu, Fu Wang, Guirong Zhang, Sufang eng Research Support, Non-U.S. Gov't England 2020/04/20 Insect Biochem Mol Biol. 2020 Jul; 122:103375. doi: 10.1016/j.ibmb.2020.103375. Epub 2020 Apr 17" |
