| Title: | milR4 and milR16 Mediated Fruiting Body Development in the Medicinal Fungus Cordyceps militaris |
| Author(s): | Shao Y; Tang J; Chen S; Wu Y; Wang K; Ma B; Zhou Q; Chen A; Wang Y; |
| Address: | "Jiangsu Key Laboratory of Food Resource Development and Quality Safe, Xuzhou University of Technology, Xuzhou, China. Jiangsu Xuzhou Technician Institute, Xuzhou, China. Jiangsu KONEN Biological Engineering Co., Ltd., Nanjing, China. Experimental Center of Clinical Research, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, China. Key Laboratory of Crop Quality Improvement of Anhui Province/Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei, China" |
| ISSN/ISBN: | 1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking) |
| Abstract: | "Cordyceps militaris readily performs sexual reproduction, thus providing a remarkably rich model for understanding the processes involved in sexual development. It could regulate expression of human genes by diet-derived miRNA-like RNAs (milRNAs). However, the study of miRNAs in C. militaris has been limited. In the present study, genes encoding Dicers, Argonautes, and RNA-dependent RNA polymerases were identified. Illumina deep sequencing was performed to characterize the milRNAs in C. militaris at asexual and sexual development stages. Total 38 milRNAs were identified and five milRNAs were validated by northern blot and qRT-PCR, out of which, 19 were specific for sexual development. Importantly, the fungi could not form fruiting bodies after disruption of milR4, while the perithecium was formed in advance after over-expression of milR4. Abnormal pale yellow fruiting body primordium, covered with abnormal primordium, was formed in the strain with miR16 disruption. Although no milR4 or milR16 target genes were identified, differential expression of many different genes involved in mycelium growth and sexual development (mating process, mating signaling, and fruiting body development) among these mutants were found. Overall, milRNAs play vital roles in sexual development in C. militaris" |
| Keywords: | Cordyceps militaris disruption microRNA-like RNAs (milRNAs) over-expression sexual development; |
| Notes: | "PubMed-not-MEDLINEShao, Ying Tang, Jin Chen, Shanglong Wu, Yonghua Wang, Kun Ma, Bin Zhou, Qiumei Chen, Anhui Wang, Yulong eng Switzerland 2019/02/15 Front Microbiol. 2019 Jan 28; 10:83. doi: 10.3389/fmicb.2019.00083. eCollection 2019" |
