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J Comp Neurol

Title:		Differential localization of NT-3 and TrpM5 in glomeruli of the olfactory bulb of mice
Author(s):		Rolen SH; Salcedo E; Restrepo D; Finger TE;
Address:		"Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, 80045"
Journal Title:		J Comp Neurol
Year:		2014
Volume:		522
Issue:		8
Page Number:		1929 - 1940
DOI:		10.1002/cne.23512
ISSN/ISBN:		1096-9861 (Electronic) 0021-9967 (Print) 0021-9967 (Linking)
Abstract:		"Olfactory sensory neurons that express transient receptor potential channel M5 (TrpM5) or neurotrophin-3 (NT-3) project to defined clusters of glomeruli situated ventrally in the main olfactory bulb. Using genetically labeled mice, we investigated whether expression of NT-3-driven betagal and TrpM5-driven GFP marked overlapping sets of glomeruli and whether expression of these markers was coordinated. Our results indicate that these markers largely characterize independent sets of olfactory sensory neuron axons and glomeruli. Further, in glomeruli in which both TrpM5-GFP and NT-3-betagal labeled axons occur, they are expressed independently. The nature of staining for these two markers also differs within glomeruli. Within each labeled TrpM5-positive glomerulus, the level of TrpM5-GFP expression was similar throughout the glomerular neuropil. In contrast, NT-3-driven betagal expression levels are heterogeneous even within heavily labeled glomeruli. In addition, a population of very small TrpM5-GFP positive glomeruli is apparent while no similar populations of NT-3-betagal glomeruli are evident. Taken together, these data suggest that TrpM5 and NT-3 characterize two largely independent receptor populations both conveying odorant information to the ventral olfactory bulb"
Keywords:		"Animals Female Male Mice Mice, Transgenic Nerve Growth Factors/*analysis/*biosynthesis Olfactory Bulb/*chemistry/*metabolism TRPM Cation Channels/*analysis/*biosynthesis olfaction, immunochemistry, mapping, semiochemical, microglomeruli;"
Notes:		"MedlineRolen, S H Salcedo, E Restrepo, D Finger, T E eng F32 DC011690/DC/NIDCD NIH HHS/ P30 DC004657/DC/NIDCD NIH HHS/ R01 DC006070/DC/NIDCD NIH HHS/ P30 DC04657/DC/NIDCD NIH HHS/ Research Support, N.I.H., Extramural 2013/11/30 J Comp Neurol. 2014 Jun 1; 522(8):1929-40. doi: 10.1002/cne.23512"