« Previous AbstractIntegrated metabolic phenotypes and gene expression profiles revealed the effect of spreading on aroma volatiles formation in postharvest leaves of green tea    Next AbstractDiscrimination of alarm pheromone (E)-beta-farnesene by aphid odorant-binding proteins »

Plant Physiol Biochem

Title:		A monoterpene synthase gene cluster of tea plant (Camellia sinensis) potentially involved in constitutive and herbivore-induced terpene formation
Author(s):		Qiao D; Tang M; Jin L; Mi X; Chen H; Zhu J; Liu S; Wei C;
Address:		"State Key Laboratory of Tea Plant Biology and Utilization / Anhui Provincial Laboratory of Tea Plant Biology and Utilization/ Key Laboratory of Tea Biology and Tea Processing of Ministry of Agriculture, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui, 230036, China; Tea Research Institute, Guizhou Academy of Agricultural Sciences, 1 Jin'nong Road, Guiyang, Guizhou, 550006, China. State Key Laboratory of Tea Plant Biology and Utilization / Anhui Provincial Laboratory of Tea Plant Biology and Utilization/ Key Laboratory of Tea Biology and Tea Processing of Ministry of Agriculture, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui, 230036, China. State Key Laboratory of Tea Plant Biology and Utilization / Anhui Provincial Laboratory of Tea Plant Biology and Utilization/ Key Laboratory of Tea Biology and Tea Processing of Ministry of Agriculture, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui, 230036, China. Electronic address: weichl@ahau.edu.cn"
Journal Title:		Plant Physiol Biochem
Year:		2022
Volume:		20220519
Issue:
Page Number:		1 - 13
DOI:		10.1016/j.plaphy.2022.05.016
ISSN/ISBN:		1873-2690 (Electronic) 0981-9428 (Linking)
Abstract:		"Monoterpenes and sesquiterpenes are the most abundant volatiles in tea plants and have dual functions in aroma quality formation and defense responses in tea plants. Terpene synthases (TPS) are the key enzymes for the synthesis of terpenes in plants; however, the functions of most of them in tea plants are still unknown. In this study, six putative terpene biosynthesis gene clusters were identified from the tea plant genome. Then we cloned three new TPS-b subfamily genes, CsTPS08, CsTPS10 and CsTPS58. In vitro enzyme assays showed that CsTPS08 and CsTPS58 are two multiple-product terpene synthases, with the former synthesizing linalool as the main product, and beta-myrcene, alpha-phellandrene, alpha-terpinolene, D-limonene, cis-beta-ocimene, trans-beta-ocimene and (4E,6Z)-allo-ocimene as minor products are also detected, while the latter catalyzing the formation of alpha-pinene and D-limonene using GPP as the substrate. No product of CsTPS10 was detected in the prokaryotic expression system, but geraniol production was detected when transiently expressed in tobacco leaves. CsTPS08 and CsTPS10 are two functional members of a monoterpene synthase gene cluster, which were significantly induced during both Ectropis oblique feeding and fresh leaf spreading treatments, suggesting that they have dual functions involved in tea plant pest defense and tea aroma quality regulation. In addition, the differences in their expression levels in different tea plant cultivars provide a possibility for the subsequent screening of tea plant resources with a specific aroma flavor. Our results deepen the understanding of terpenoid synthesis in tea plants"
Keywords:		*Alkyl and Aryl Transferases/genetics/metabolism *Camellia sinensis/metabolism Herbivory Intramolecular Lyases Limonene/metabolism Multigene Family Plant Proteins/genetics/metabolism Tea Terpenes/metabolism Biosynthetic gene clusters Camellia sinensis Ger;
Notes:		"MedlineQiao, Dahe Tang, Mengsha Jin, Ling Mi, Xiaozeng Chen, Hongrong Zhu, Junyan Liu, Shengrui Wei, Chaoling eng France 2022/05/26 Plant Physiol Biochem. 2022 Aug 1; 184:1-13. doi: 10.1016/j.plaphy.2022.05.016. Epub 2022 May 19"