| Title: | Light induces an increasing release of benzyl nitrile against diurnal herbivore Ectropis grisescens Warren attack in tea (Camellia sinensis) plants |
| Author(s): | Qian J; Liao Y; Jian G; Jia Y; Zeng L; Gu D; Li H; Yang Y; |
| Address: | "Guangdong Provincial Key Laboratory of Applied Botany & Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China. South China National Botanical Garden, Guangzhou, China. University of Chinese Academy of Sciences, Beijing, China. Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Guangzhou, China" |
| ISSN/ISBN: | 1365-3040 (Electronic) 0140-7791 (Linking) |
| Abstract: | "Herbivore-induced plant volatiles (HIPVs) are critical compounds that directly or indirectly regulate the tritrophic interactions among herbivores, natural enemies and plants. The synthesis and release of HIPVs are regulated by many biotic and abiotic factors. However, the mechanism by which multiple factors synergistically affect HIPVs release remains unclear. Tea plant (Camellia sinensis) is the object of this study because of its rich and varied volatile metabolites. In this study, benzyl nitrile was released from herbivore-attacked tea plants more in the daytime than at night, which was consistent with the feeding behaviour of tea geometrid (Ectropis grisescens Warren) larvae. The Y-tube olfactometer assay and insect resistance analysis revealed that benzyl nitrile can repel tea geometrid larvae and inhibit their growth. On the basis of enzyme activities in transiently transformed Nicotiana benthamiana plants, CsCYP79 was identified as a crucial regulator in the benzyl nitrile biosynthetic pathway. Light signalling-related transcription factor CsPIF1-like and the jasmonic acid (JA) signalling-related transcription factor CsMYC2 serve as the activator of CsCYP79 under light and damage conditions. Our study revealed that light (abiotic factor) and herbivore-induced damage (biotic stress) synergistically regulate the synthesis and release of benzyl nitrile to protect plants from diurnal herbivorous tea geometrid larvae" |
| Keywords: | Animals *Camellia sinensis/metabolism Herbivory Nitriles/metabolism *Lepidoptera Larva/physiology Tea/metabolism Camellia sinensis Ectropis grisescens anti-insect benzyl nitrile jasmonic acid light; |
| Notes: | "MedlineQian, Jiajia Liao, Yinyin Jian, Guotai Jia, Yongxia Zeng, Lanting Gu, Dachuan Li, Hanxiang Yang, Yuhua eng 2023B0202120001/Key-Area Research and Development Program of Guangdong Province/ 2021M693221/China Postdoctoral Science Foundation/ 2021000064/Special Research Assistant Program of Chinese Academy of Sciences/ ZDBS-LY-SM032/Basic Frontier Science Research Program of Chinese Academy of Sciences/ 2021A1515011311/Guangdong Basic and Applied Basic Research Foundation/ 2020QNRC001/Young Elite Scientists Sponsorship Program by China Association for Science and Technology/ E225052A01/Talent Technology Support Program of Chinese Academy of Sciences/ 2023KJ120/Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams/ E336030011/Guangdong Forestry Bureau (Key Laboratory of ex situ plant protection and utilization in South China)/ 2023/08/09 Plant Cell Environ. 2023 Nov; 46(11):3464-3480. doi: 10.1111/pce.14687. Epub 2023 Aug 8" |
