| Title: | Emission Timetable and Quantitative Patterns of Wound-Induced Volatiles Across Different Leaf Damage Treatments in Aspen (Populus Tremula) |
| Author(s): | Portillo-Estrada M; Kazantsev T; Talts E; Tosens T; Niinemets U; |
| Address: | "Department of Plant Physiology, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1, 51014, Tartu, Estonia. Miguel.PortilloEstrada@uantwerpen.be. Centre of Excellence PLECO (Plant and Vegetation Ecology), Department of Biology, University of Antwerp, Universiteitsplein 1, B-2610, Wilrijk, Belgium. Miguel.PortilloEstrada@uantwerpen.be. Department of Plant Physiology, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1, 51014, Tartu, Estonia. Taras.Kazantsev@emu.ee. Department of Plant Physiology, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1, 51014, Tartu, Estonia. Eero.Talts@emu.ee. Department of Plant Physiology, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1, 51014, Tartu, Estonia. Tiina.Tosens@emu.ee. Department of Plant Physiology, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1, 51014, Tartu, Estonia. Ylo.Niinemets@emu.ee. Estonian Academy of Sciences, Kohtu 6, 10130, Tallinn, Estonia. Ylo.Niinemets@emu.ee" |
| DOI: | 10.1007/s10886-015-0646-y |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Print) 0098-0331 (Linking) |
| Abstract: | "Plant-feeding herbivores can generate complex patterns of foliar wounding, but it is unclear how wounding-elicited volatile emissions scale with the severity of different wounding types, and there is no common protocol for wounding experiments. We investigated the rapid initial response to wounding damage generated by different numbers of straight cuts and punctures through leaf lamina as well as varying area of lamina squeezing in the temperate deciduous tree Populus tremula. Wounding-induced volatile emission time-courses were continuously recorded by a proton-transfer-reaction time-of-flight mass-spectrometer. After the mechanical wounding, an emission cascade was rapidly elicited resulting in sequential emissions of key stress volatiles methanol, acetaldehyde, and volatiles of the lipoxygenase pathway, collectively constituting more than 97% of the total emission. The maximum emission rates, reached after one to three minutes after wounding, and integrated emissions during the burst were strongly correlated with the severity in all damage treatments. For straight cuts and punch hole treatments, the emissions per cut edge length were constant, indicating a direct proportionality. Our results are useful for screening wounding-dependent emission capacities" |
| Keywords: | *Herbivory Mass Spectrometry Plant Leaves/*metabolism Populus/*metabolism Trees/metabolism Volatile Organic Compounds/*metabolism Abiotic stress Green volatiles Hexenal LOX products Proton-transfer-reaction; |
| Notes: | "MedlinePortillo-Estrada, Miguel Kazantsev, Taras Talts, Eero Tosens, Tiina Niinemets, Ulo eng 322603/ERC_/European Research Council/International Research Support, Non-U.S. Gov't 2015/11/08 J Chem Ecol. 2015 Dec; 41(12):1105-17. doi: 10.1007/s10886-015-0646-y. Epub 2015 Nov 6" |
