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Fungal Genet Biol

Title:		Analysis of gene expression related to polyamine concentration and dimorphism induced in ornithine decarboxylase (odc) and spermidine synthase (spd) Ustilago maydis mutants
Author(s):		Perez-Rodriguez F; Valdes-Santiago L; Garcia-Chavez JN; Castro-Guillen JL; Ruiz-Herrera J;
Address:		"Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Carretera Irapuato Silao Km 12.5, Irapuato C.P. 36821, Mexico; Departamento de Ciencias Biomedicas, Universidad Quetzalcoatl Irapuato, Irapuato, Guanajuato C.P. 36615, Mexico. Tecnologico Nacional de Mexico / Instituto Tecnologico Superior de Irapuato / Ingenieria Bioquimica, Carretera Irapuato-Silao Km 12.5, Irapuato C.P. 36821, Mexico. Electronic address: laura.vs@irapuato.tecnm.mx. Langebio, Unidad de Genomica Avanzada, Centro de Investigacion y de estudios Avanzados del Instituto Politecnico Nacional, Carretera Irapuato Silao Km 12.5, Irapuato C.P. 36821, Mexico. Electronic address: j.noe.garcia.c@gmail.com. Tecnologico Nacional de Mexico / Instituto Tecnologico Superior de Irapuato / Ingenieria Bioquimica, Carretera Irapuato-Silao Km 12.5, Irapuato C.P. 36821, Mexico. Electronic address: jlcg.tekk.el@gmail.com. Departamento de Ingenieria Genetica, Unidad Irapuato, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Carretera Irapuato Silao Km 12.5, Irapuato C.P. 36821, Mexico. Electronic address: jose.ruiz@cinvestav.mx"
Journal Title:		Fungal Genet Biol
Year:		2023
Volume:		20230328
Issue:
Page Number:		103792 -
DOI:		10.1016/j.fgb.2023.103792
ISSN/ISBN:		1096-0937 (Electronic) 1087-1845 (Linking)
Abstract:		"Polyamines are ubiquitous small organic cations, and their roles as regulators of several cellular processes are widely recognized. They are implicated in the key stages of the fungal life cycle. Ustilago maydis is a phytopathogenic fungus, the causal agent of common smut of maize and a model system to understand dimorphism and virulence. U. maydis grows in yeast form at pH 7 and it can develop its mycelial form in vitro at pH 3. Deltaodc mutants that are unable to synthesize polyamines, grow as yeast at pH 3 with a low putrescine concentration, and to complete its dimorphic transition high putrescine concentration is require. Deltaspd mutants require spermidine to grow and cannot form mycelium at pH 3. In this work, the increased expression of the mating genes, mfa1 and mfa2, on Deltaodc mutants, was related to high putrescine concentration. Global gene expression analysis comparisons of Deltaodc and Deltaspd U. maydis mutants indicated that 2,959 genes were differentially expressed in the presence of exogenous putrescine at pH 7 and 475 genes at pH 3. While, in Deltaspd mutant, the expression of 1,426 genes was affected by exogenous spermine concentration at pH 7 and 11 genes at pH 3. Additionally, we identified 28 transcriptional modules with correlated expression during seven tested conditions: mutant genotype, morphology (yeast, and mycelium), pH, and putrescine or spermidine concentration. Furthermore, significant differences in transcript levels were noted for genes in modules relating to pH and genotype genes involved in ribosome biogenesis, mitochondrial oxidative phosphorylation, N-glycan synthesis, and Glycosylphosphatidylinositol (GPI)-anchor. In summary, our results offer a valuable tool for the identification of potential factors involved in phenomena related to polyamines and dimorphism"
Keywords:		*Polyamines/metabolism Putrescine/metabolism/pharmacology Spermidine/metabolism Ornithine Decarboxylase/genetics/metabolism Spermidine Synthase/genetics Saccharomyces cerevisiae/genetics Sex Characteristics Gene Expression Lipoproteins/genetics Pheromones;
Notes:		"MedlinePerez-Rodriguez, Fernando Valdes-Santiago, Laura Garcia-Chavez, Jorge Noe Castro-Guillen, Jose Luis Ruiz-Herrera, Jose eng Research Support, Non-U.S. Gov't 2023/03/31 Fungal Genet Biol. 2023 May; 166:103792. doi: 10.1016/j.fgb.2023.103792. Epub 2023 Mar 28"