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Insect Mol Biol

Title:		Cytochrome P450 CYP6DA2 regulated by cap 'n'collar isoform C (CncC) is associated with gossypol tolerance in Aphis gossypii Glover
Author(s):		Peng T; Pan Y; Gao X; Xi J; Zhang L; Yang C; Bi R; Yang S; Xin X; Shang Q;
Address:		"College of Plant Science, Jilin University, Changchun, China. Department of Entomology, China Agricultural University, Beijing, China. Department of Entomology, Jilin Agricultural University, Changchun, China"
Journal Title:		Insect Mol Biol
Year:		2016
Volume:		20160323
Issue:		4
Page Number:		450 - 459
DOI:		10.1111/imb.12230
ISSN/ISBN:		1365-2583 (Electronic) 0962-1075 (Linking)
Abstract:		"Cotton plants accumulate phytotoxins, such as gossypol and related sesquiterpene aldehydes, to resist insect herbivores. The survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of cytochrome P450. Gossypol has an antibiotic effect on Aphis gossypii, and as the concentrations of gossypol were increased in the present study, the mortality of cotton aphids increased from 4 to 28%. The fecundity of the cotton aphids exposed to gossypol was also significantly reduced compared with the control. The transcriptional levels of CYP6DA2 in cotton aphids were significantly induced when exposed to gossypol, and knockdown of the CYP6DA2 transcripts by RNA interference (RNAi) significantly increased the toxicity of gossypol to cotton aphids. To further understand the gossypol regulatory cascade, the 5'-flanking promoter sequences of CYP6DA2 were isolated with a genome walker, and the promoter was very active and was inducible by gossypol. Co-transfection of the cap 'n' collar isoform C (CncC) and CYP6DA2 promoters dramatically increased the expression of CYP6DA2, and suppression of the CncC transcripts by RNAi significantly decreased the expression levels of CYP6DA2, and significantly increased the toxicity of gossypol to cotton aphids. Thus, the transcriptional regulation of CYP6DA2 involved the transcriptional factor CncC"
Keywords:		"Amino Acid Sequence Animals Aphids/*genetics/metabolism Base Sequence Cloning, Molecular Cytochrome P-450 Enzyme System/*genetics/metabolism DNA, Complementary/genetics/metabolism Fertility *Gene Expression Regulation Gossypium/chemistry Gossypol/metaboli;"
Notes:		"MedlinePeng, T Pan, Y Gao, X Xi, J Zhang, L Yang, C Bi, R Yang, S Xin, X Shang, Q eng Research Support, Non-U.S. Gov't England 2016/03/24 Insect Mol Biol. 2016 Aug; 25(4):450-9. doi: 10.1111/imb.12230. Epub 2016 Mar 23"