« Previous Abstract"Chemical and volatile composition, and microbial communities in edible purple flowers (Torenia fournieri F. Lind.) cultivated in different organic systems"    Next Abstract"Comparative application of solid-phase microextraction fibre assemblies and semi-permeable membrane devices as passive air samplers for semi-volatile chlorinated organic compounds. A case study on the landfill 'Grube Antonie' in Bitterfeld, Germany" »

Front Cell Neurosci

Title:		NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements
Author(s):		Pascarella G; Lazarevic D; Plessy C; Bertin N; Akalin A; Vlachouli C; Simone R; Faulkner GJ; Zucchelli S; Kawai J; Daub CO; Hayashizaki Y; Lenhard B; Carninci P; Gustincich S;
Address:		"Area of Neuroscience, International School for Advanced Studies (SISSA) Trieste, Italy ; RIKEN Yokohama Institute, Center for Life Science Technologies, Division of Genomic Technologies Tsurumi-ku, Yokohama, Japan. Area of Neuroscience, International School for Advanced Studies (SISSA) Trieste, Italy ; Cluster in Biomedicine (CBM), AREA Science Park Trieste, Italy. RIKEN Yokohama Institute, Center for Life Science Technologies, Division of Genomic Technologies Tsurumi-ku, Yokohama, Japan. Bergen Center for Computational Science - Computational Biology Unit and Sars Centre for Marine Molecular Biology, University of Bergen Bergen, Norway. Area of Neuroscience, International School for Advanced Studies (SISSA) Trieste, Italy. Cancer Biology Program, Mater Medical Research Institute South Brisbane, QLD, Australia ; School of Biomedical Sciences, University of Queensland Brisbane, QLD, Australia. Area of Neuroscience, International School for Advanced Studies (SISSA) Trieste, Italy ; Department of Health Sciences, University of Eastern Piedmont 'A. Avogadro,' Novara, Italy"
Journal Title:		Front Cell Neurosci
Year:		2014
Volume:		20140218
Issue:
Page Number:		41 -
DOI:		10.3389/fncel.2014.00041
ISSN/ISBN:		1662-5102 (Print) 1662-5102 (Electronic) 1662-5102 (Linking)
Abstract:		"By coupling laser capture microdissection to nanoCAGE technology and next-generation sequencing we have identified the genome-wide collection of active promoters in the mouse Main Olfactory Epithelium (MOE). Transcription start sites (TSSs) for the large majority of Olfactory Receptors (ORs) have been previously mapped increasing our understanding of their promoter architecture. Here we show that in our nanoCAGE libraries of the mouse MOE we detect a large number of tags mapped in loci hosting Type-1 and Type-2 Vomeronasal Receptors genes (V1Rs and V2Rs). These loci also show a massive expression of Long Interspersed Nuclear Elements (LINEs). We have validated the expression of selected receptors detected by nanoCAGE with in situ hybridization, RT-PCR and qRT-PCR. This work extends the repertory of receptors capable of sensing chemical signals in the MOE, suggesting intriguing interplays between MOE and VNO for pheromone processing and positioning transcribed LINEs as candidate regulatory RNAs for VRs expression"
Keywords:		Moe V1Rs V2Rs Vno main olfactory epithelium vomeronasal organ vomeronasal receptors;neuroscience;
Notes:		"PubMed-not-MEDLINEPascarella, Giovanni Lazarevic, Dejan Plessy, Charles Bertin, Nicolas Akalin, Altuna Vlachouli, Christina Simone, Roberto Faulkner, Geoffrey J Zucchelli, Silvia Kawai, Jun Daub, Carsten O Hayashizaki, Yoshihide Lenhard, Boris Carninci, Piero Gustincich, Stefano eng Switzerland 2014/03/07 Front Cell Neurosci. 2014 Feb 18; 8:41. doi: 10.3389/fncel.2014.00041. eCollection 2014"