| Title: | Bisphenol A damages testicular junctional proteins transgenerationally in mice |
| Author(s): | Adegoke EO; Rahman MS; Amjad S; Pang WK; Ryu DY; Park YJ; Pang MG; |
| Address: | "Department of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, Gyeonggi-do, 17546, Republic of Korea. Department of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, Gyeonggi-do, 17546, Republic of Korea. Electronic address: mgpang@cau.ac.kr" |
| DOI: | 10.1016/j.envpol.2022.119067 |
| ISSN/ISBN: | 1873-6424 (Electronic) 0269-7491 (Linking) |
| Abstract: | "Testicular junctions are pivotal to male fertility and regulated by constituent proteins. Increasing evidence suggests that environmental chemicals, including bisphenol A (BPA), may impact these proteins, but whether the impacts persist for generations is not yet known. Here, we investigate the effect of BPA (a ubiquitous endocrine-disrupting chemical) on testis and sperm functions and whether the effects are transferred to subsequent generations. Male mice (F0) were exposed to corn oil (Control) or 5 or 50 mg BPA/kg body weight/day from 6 to 12 weeks of age. The F0 were mated with wild-type females to produce the first filial (F1) generation. F2 and F3 were produced using similar procedures. Our results showed that BPA doses decreased the levels of some junctional proteins partly via binding with estrogen receptors (ERalpha and Erbeta), upregulation of p-ERK1/2, P85, p-JNK and activation of p38 mitogen-activated protein kinase signaling. Consequently, testicular histological abnormalities, disrupted spermatogenesis, decreased sperm count, and inability to fertilize eggs were observed in mice exposed to BPA. These effects were transferred to successive generations (F2), partly through DNA methylation, but mostly alleviated in F3 males. Our findings suggest that paternal exposure to chemicals promoting alteration of testicular junctional proteins and its transgenerational inheritance is a key component of the origin of male reproductive health problems" |
| Keywords: | Animals Benzhydryl Compounds/metabolism *Endocrine Disruptors/metabolism Female Male Mice Phenols/metabolism *Prenatal Exposure Delayed Effects/metabolism Testis DNA methylation Environmental chemical Fertility Reproductive health; |
| Notes: | "MedlineAdegoke, Elikanah Olusayo Rahman, Md Saidur Amjad, Shereen Pang, Won-Ki Ryu, Do-Yeal Park, Yoo-Jin Pang, Myung-Geol eng England 2022/03/02 Environ Pollut. 2022 Jun 1; 302:119067. doi: 10.1016/j.envpol.2022.119067. Epub 2022 Feb 26" |
