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FEMS Yeast Res

Title:		Kluyveromyces lactis sexual pheromones. Gene structures and cellular responses to alpha-factor
Author(s):		Ongay-Larios L; Navarro-Olmos R; Kawasaki L; Velazquez-Zavala N; Sanchez-Paredes E; Torres-Quiroz F; Coello G; Coria R;
Address:		"Unidad de Biologia Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Mexico"
Journal Title:		FEMS Yeast Res
Year:		2007
Volume:		20070516
Issue:		5
Page Number:		740 - 747
DOI:		10.1111/j.1567-1364.2007.00249.x
ISSN/ISBN:		1567-1356 (Print) 1567-1356 (Linking)
Abstract:		"The Kluyveromyces lactis genes for sexual pheromones have been analyzed. The alpha-factor gene encodes a predicted polypeptide of 187 amino acid residues containing four tridecapeptide repeats (WSWITLRPGQPIF). A nucleotide blast search of the entire K. lactis genome sequence allowed the identification of the nonannotated putative a-pheromone gene that encodes a predicted protein of 33 residues containing one copy of the dodecapeptide a-factor (WIIPGFVWVPQC). The role of the K. lactis structural genes KlMFalpha1 and KlMFA1 in mating has been investigated by the construction of disruption mutations that totally eliminate gene functions. Mutants of both alleles showed sex-dependent sterility, indicating that these are single-copy genes and essential for mating. MATalpha, Klsst2 mutants, which, by analogy to Saccharomyces cerevisiae, are defective in Galpha-GTPase activity, showed increased sensitivity to synthetic alpha-factor and increased capacity to mate. Additionally, Klbar1 mutants (putatively defective in alpha-pheromone proteolysis) showed delay in mating but sensitivity to alpha-pheromone. From these results, it can be deduced that the K. lactis MATa cell produces the homolog of the S. cerevisiaealpha-pheromone, whereas the MATalpha cell produces the a-pheromone"
Keywords:		"Amino Acid Sequence Base Sequence Fungal Proteins/genetics/metabolism/pharmacology Gene Deletion *Gene Expression Regulation, Fungal Genes, Fungal Kluyveromyces/*drug effects/*genetics/metabolism/physiology Mating Factor Molecular Sequence Data Peptides/c;"
Notes:		"MedlineOngay-Larios, Laura Navarro-Olmos, Rocio Kawasaki, Laura Velazquez-Zavala, Nancy Sanchez-Paredes, Edith Torres-Quiroz, Francisco Coello, Gerardo Coria, Roberto eng Research Support, Non-U.S. Gov't England 2007/05/18 FEMS Yeast Res. 2007 Aug; 7(5):740-7. doi: 10.1111/j.1567-1364.2007.00249.x. Epub 2007 May 16"