| Title: | Analysis of honeybush tea (Cyclopia spp.) volatiles by comprehensive two-dimensional gas chromatography using a single-stage thermal modulator |
| Author(s): | Ntlhokwe G; Tredoux AGJ; Gorecki T; Edwards M; Vestner J; Muller M; Erasmus L; Joubert E; Christel Cronje J; de Villiers A; |
| Address: | "Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada. Center of Analytical Chemistry & Microbiology, Department of Microbiology and Biochemistry, Hochschule Geisenheim University, Von-Lade-Str. 1, 65366, Geisenheim, Germany. Universite de Bordeaux, ISVV, EA 4577, Unite de recherche OEnologie, 33882, Villenave d'Ornon, France. INRA, ISVV, USC 1366 OEnologie, 33882, Villenave d'Ornon, France. Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC), Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7566, South Africa. Department of Forest and Wood Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. ajdevill@sun.ac.za" |
| DOI: | 10.1007/s00216-017-0360-4 |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Linking) |
| Abstract: | "The applicability of comprehensive two-dimensional gas chromatography (GCxGC) using a single-stage thermal modulator was explored for the analysis of honeybush tea (Cyclopia spp.) volatile compounds. Headspace solid phase micro-extraction (HS-SPME) was used in combination with GCxGC separation on a non-polar x polar column set with flame ionisation (FID) detection for the analysis of fermented Cyclopia maculata, Cyclopia subternata and Cyclopia genistoides tea infusions of a single harvest season. Method optimisation entailed evaluation of the effects of several experimental parameters on the performance of the modulator, the choice of columns in both dimensions, as well as the HS-SPME extraction fibre. Eighty-four volatile compounds were identified by co-injection of reference standards. Principal component analysis (PCA) showed clear differentiation between the species based on their volatile profiles. Due to the highly reproducible separations obtained using the single-stage thermal modulator, multivariate data analysis was simplified. The results demonstrate both the complexity of honeybush volatile profiles and the potential of GCxGC separation in combination with suitable data analysis techniques for the investigation of the relationship between sensory properties and volatile composition of these products. The developed method therefore offers a fast and inexpensive methodology for the profiling of honeybush tea volatiles. Graphical abstract Surface plot obtained for the GCxGC-FID analysis of honeybush tea volatiles" |
| Keywords: | "Fabaceae/*chemistry Gas Chromatography-Mass Spectrometry/instrumentation/*methods Multivariate Analysis Principal Component Analysis Solid Phase Microextraction/methods Teas, Herbal/*analysis Temperature Volatile Organic Compounds/*analysis Comprehensive;" |
| Notes: | "MedlineNtlhokwe, Gaalebalwe Tredoux, Andreas G J Gorecki, Tadeusz Edwards, Matthew Vestner, Jochen Muller, Magdalena Erasmus, Lene Joubert, Elizabeth Christel Cronje, J de Villiers, Andre eng Germany 2017/04/19 Anal Bioanal Chem. 2017 Jul; 409(17):4127-4138. doi: 10.1007/s00216-017-0360-4. Epub 2017 Apr 17" |
