| Title: | Dynamic changes in virus-induced volatiles in cotton modulate the orientation and oviposition behavior of the whitefly Bemisia tabaci |
| Author(s): | Nebapure SM; Shankarganesh K; Rajna S; Naga KC; Pandey D; Gambhir S; Praveen KV; Subramanian S; |
| Address: | "ICAR-Indian Agricultural Research Institute, New Delhi, India. ICAR-Central Institute for Cotton Research, Regional Station, Coimbatore, India. ICAR-Central Potato Research Institute, Shimla, India" |
| DOI: | 10.3389/fphys.2022.1017948 |
| ISSN/ISBN: | 1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking) |
| Abstract: | "Manipulation of insect vector behavior by virus-induced plant volatiles is well known. But how the viral disease progression alters the plant volatiles and its effect on vector behavior remains less explored. Our studies tracked changes in volatile profile in progressive infection stages of cotton leaf curl virus (CLCuV) infected plants and their effect on B. tabaci behavior. Significant differences in virus titers were noticed between progressive infection stages showing distinct symptoms. Whiteflies initially settled on CLCuV infected plants, but their preference was shifted to healthy plants over time. GC-MS analysis revealed subtle quantitative/qualitative changes in volatile organic compounds (VOCs) between the healthy and selected CLCuV infection stages. VOCs such as hexanal, (E)-2-hexen-1-ol, (+)-alpha-pinene, (-)-beta-pinene, (Z)-3-hexen-1-ol, (+)-sylvestrene, and (1S,2E,6E, 10R)-3,7,11,11-tetramethylbicycloundeca-2,6-diene (Bicyclogermacrene) were associated with the infection stage showing upward curling of leaves; (E)-2-hexen-1-ol, beta-myrcene, beta-ocimene, and copaene were associated with the infection stage showing downward curling. Validation studies with eight synthetic VOCs indicated that gamma-terpinene elicited attraction to B. tabaci (Olfactometric Preference Index (OPI) = 1.65), while beta-ocimene exhibited strong repellence (OPI = 0.64) and oviposition reduction (66.01%-92.55%). Our studies have demonstrated that progression of CLCuV disease in cotton was associated with dynamic changes in volatile profile which influences the behavioural responses of whitefly, B.tabaci. Results have shown that VOCs such as (+)-alpha-pinene, (-)-beta-pinene gamma-Terpinene, alpha-guaiene; 4- hydroxy- 4 methyl-2- pentanone and beta-ocimene emitted from Begomovirus infected plants could be the driving force for early attraction and later repellence/oviposition deterrence of B. tabaci on virus-infected plants. The findings of this study offer scope for the management of whitefly, B. tabaci through semiochemicals" |
| Keywords: | cotton leaf curl virus interactions orientation oviposition volatile organic compounds whitefly; |
| Notes: | "PubMed-not-MEDLINENebapure, Suresh M Shankarganesh, Karuppan Rajna, Salim Naga, Kailash Chandra Pandey, Dheerendra Gambhir, Shubham Praveen, Koovalamkadu Velayudhan Subramanian, Sabtharishi eng Switzerland 2022/10/28 Front Physiol. 2022 Oct 10; 13:1017948. doi: 10.3389/fphys.2022.1017948. eCollection 2022" |
