| Title: | Fruit volatilome profiling through GC x GC-ToF-MS and gene expression analyses reveal differences amongst peach cultivars in their response to cold storage |
| Author(s): | Muto A; Muller CT; Bruno L; McGregor L; Ferrante A; Chiappetta AAC; Bitonti MB; Rogers HJ; Spadafora ND; |
| Address: | "Department of Biology, Ecology and Earth Sciences, University of Calabria, Via Ponte P. Bucci 6b, 87036, Arcavacata Di Rende, Cosenza, Italy. School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AT, UK. SepSolve Analytical Ltd, Swan Court, Hampton, PE7 8GX, Peterborough, UK. Department of Agricultural and Environmental Science-Production, Landscape, Agroenergy, Universita Degli Studi Di Milano, Via Celoria 2, 20133, Milan, Italy. Department of Biology, Ecology and Earth Sciences, University of Calabria, Via Ponte P. Bucci 6b, 87036, Arcavacata Di Rende, Cosenza, Italy. nspadafora@markes.com. School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AT, UK. nspadafora@markes.com. Markes International, Gwaun Elai Medi-Science Campus, Llantrisant, Cardiff, UK. nspadafora@markes.com" |
| DOI: | 10.1038/s41598-020-75322-z |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Peaches have a short shelf life and require chilling during storage and transport. Peach aroma is important for consumer preference and determined by underlying metabolic pathways and gene expression. Differences in aroma (profiles of volatile organic compounds, VOCs) have been widely reported across cultivars and in response to cold storage. However, few studies used intact peaches, or used equilibrium sampling methods subject to saturation. We analysed VOC profiles using TD-GC x GC-ToF-MS and expression of 12 key VOC pathway genes of intact fruit from six cultivars (three peaches, three nectarines) before and after storage at 1 degrees C for 7 days including 36 h shelf life storage at 20 degrees C. Two dimensional GC (GC x GC) significantly enhances discrimination of thermal desorption gas chromatography time-of-flight mass spectrometry (TD-GC-ToF-MS) and detected a total of 115 VOCs. A subset of 15 VOCs from analysis with Random Forest discriminated between cultivars. Another 16 VOCs correlated strongly with expression profiles of eleven key genes in the lipoxygenase pathway, and both expression profiles and VOCs discriminated amongst cultivars, peach versus nectarines and between treatments. The cultivar-specific response to cold storage underlines the need to understand more fully the genetic basis for VOC changes across cultivars" |
| Keywords: | "Chromatography, Gas Cold Temperature/adverse effects *Food Storage Fruit/*genetics/metabolism/physiology Gas Chromatography-Mass Spectrometry Gene Expression Profiling Prunus persica/*genetics/metabolism/physiology Transcriptome Volatile Organic Compounds;" |
| Notes: | "MedlineMuto, Antonella Muller, Carsten T Bruno, Leonardo McGregor, Laura Ferrante, Antonio Chiappetta, Adriana Ada Ceverista Bitonti, Maria Beatrice Rogers, Hilary J Spadafora, Natasha Damiana eng Research Support, Non-U.S. Gov't England 2020/10/29 Sci Rep. 2020 Oct 27; 10(1):18333. doi: 10.1038/s41598-020-75322-z" |
