| Title: | "Enzymatic, non enzymatic antioxidants and glucose metabolism enzymes response differently against metal stress in muscles of three fish species depending on different feeding niche" |
| Author(s): | Moniruzzaman M; Kumar S; Das D; Sarbajna A; Chakraborty SB; |
| Address: | "Fish Endocrinology Research Unit, Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, India. Department of Zoology, Surendranath College, Kolkata, 700009, India. Electronic address: aditymukhopadhyay@gmail.com. Fish Endocrinology Research Unit, Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, India. Electronic address: sumanbc76@gmail.com" |
| DOI: | 10.1016/j.ecoenv.2020.110954 |
| ISSN/ISBN: | 1090-2414 (Electronic) 0147-6513 (Linking) |
| Abstract: | "Current study aims to determine difference in metal accumulation pattern in muscle of Liza parsia (pelagic, omnivore), Amblypharyngodon mola (surface feeder, herbivore) and Mystus gulio (benthic, carnivore) depending on their niche and feeding habit and how it affects the endogenous antioxidants and glucose metabolism in fish muscle. Fishes were collected from Malancha, Diamond Harbour and Chandanpiri, West Bengal, India. Concentrations of lead, zinc, cadmium, chromium were measured in water, sediment and fish muscle. Metal pollution index (MPI) and bioconcentration factor (BCF) was calculated to evaluate the ability of fish to accumulate specific metals in muscle tissue from the aquatic environment. Metal concentrations were found signi fi cantly higher (P < 0.05) in water, sediment, fi sh muscles from Malancha than Chandanpiri and Diamond Harbour. L. parsia (MPI: 0.4-1.6) showed highest metal deposition in their muscle followed by A. mola (MPI: 0.37-1.38) and M. gulio (MPI: 0.2-1.2). Malondealdehyde, superoxide dismutase, catalase, glutathione S transferase, glutathione reductase and cortisol levels increased in case of L. parsia from Malancha and Chandanpiri. Succinate dehydrogenase, lactate dehydrogenase, Ca(+2) ATPase and cytochrome C oxidase levels were significantly (P < 0.05) lower at Malancha and Chandanpiri than Diamond Harbour. Heat shock protein (HSP70) expression was significantly (P < 0.05) higher in all fish species at Malancha followed by Chandanpiri and Diamond Harbour. Glucose, glycogen, hexokinase, phosphofructokinase and glycogen phosphorylase levels varied between sites and selected fish species. Serum cortisol level was measured and found to be the highest in L. parsia from Malancha (2.94 +/- 0.12 ng/ml) and the lowest in M. gulio from Diamond Harbour (0.7 +/- 0.05 ng/ml). The results indicate that metal toxicity alters antioxidant levels, oxidative status and energy production in fish in species specific manner. Our results also indicate that Mystus has the highest degree of adaptability in response to metal toxicity possibly due to its specific food habit and niche position. Therefore, it can be concluded that maintenance of oxidative and metabolic status to combat metal-induced oxidative load will be helpful for the fishes to acquire better resistance under such eco-physiological stress. Alteration of niche and interactive segregation in aquatic organism may be one of the key modulator of resistance against such stress" |
| Keywords: | "Animals Antioxidants/*metabolism Catalase/metabolism Cyprinidae/*metabolism/physiology Feeding Behavior/*physiology Glucose/*metabolism Glutathione Reductase/metabolism Glutathione Transferase/metabolism India Metals, Heavy/metabolism/*toxicity Muscles/*d;" |
| Notes: | "MedlineMoniruzzaman, Mahammed Kumar, Saheli Das, Debjit Sarbajna, Adity Chakraborty, Suman Bhusan eng Netherlands 2020/08/18 Ecotoxicol Environ Saf. 2020 Oct 1; 202:110954. doi: 10.1016/j.ecoenv.2020.110954. Epub 2020 Jul 11" |
