| Title: | Determination of the disulfide array of the first inducible antifungal peptide from insects: drosomycin from Drosophila melanogaster |
| Author(s): | Michaut L; Fehlbaum P; Moniatte M; Van Dorsselaer A; Reichhart JM; Bulet P; |
| Address: | "Institut de Biologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique, Reponse Immunitaire et Developpement chezles Insectes, Strasbourg, France" |
| DOI: | 10.1016/0014-5793(96)00992-1 |
| ISSN/ISBN: | 0014-5793 (Print) 0014-5793 (Linking) |
| Abstract: | "Drosomycin is a 44-residue antifungal peptide with four intramolecular disulfide bridges which have been isolated from immune-challenged Drosophila. To produce adequate amounts of this peptide for 3D-structure analysis, studies on the mode of action and activity spectrum, we expressed a synthetic cDNA in Saccharomyces cerevisiae. For this purpose, we used the mating factor alpha gene and concomitantly overexpressed the KEX2 gene to increase the yield of fully processed drosomycin. Using a combination of Edman degradation and mass spectrometry, we show that drosomycin shares the same array of intramolecular disulfide bridges than plant defensins, in addition to their sequence similarities" |
| Keywords: | "Amino Acid Sequence Animals Antifungal Agents/*chemistry/isolation & purification Base Sequence DNA, Recombinant Disulfides/*chemistry *Drosophila Proteins Drosophila melanogaster/*chemistry Gene Expression Genetic Vectors/genetics *Insect Proteins Mating;" |
| Notes: | "MedlineMichaut, L Fehlbaum, P Moniatte, M Van Dorsselaer, A Reichhart, J M Bulet, P eng Research Support, Non-U.S. Gov't England 1996/10/14 FEBS Lett. 1996 Oct 14; 395(1):6-10. doi: 10.1016/0014-5793(96)00992-1" |
