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Insects

Title:		Identification and Functional Characterization of General Odorant Binding Proteins in Orthaga achatina
Author(s):		Ma Y; Li Y; Wei ZQ; Hou JH; Si YX; Zhang J; Dong SL; Yan Q;
Address:		"Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China"
Journal Title:		Insects
Year:		2023
Volume:		20230222
Issue:		3
Page Number:		-
DOI:		10.3390/insects14030216
ISSN/ISBN:		2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking)
Abstract:		"The olfactory system in insects are crucial for recognition of host plants and oviposition sites. General odorant binding proteins (GOBPs) are thought to be involved in detecting odorants released by host plants. Orthaga achatina (Lepidoptera: Pyralidae) is one of the most serious pests of camphor trees, Cinnamomum camphora (L.) Presl, an important urban tree species in southern China. In this study, we study the GOBPs of O. achatina. Firstly, two full-length GOBP genes (OachGOBP1 and OachGOBP2) were successfully cloned according to transcriptome sequencing results, and real-time quantitative PCR measurements showed that both GOBP genes were specifically expressed in the antennae of both sexes, proposing their important roles in olfaction. Then, both GOBP genes were heterologous expressed in Escherichia coli and fluorescence competitive binding assays were conducted. The results showed that OachGOBP1 could bind Farnesol (K(i) = 9.49 muM) and Z11-16: OH (K(i) = 1.57 muM). OachGOBP2 has a high binding affinity with two camphor plant volatiles (Farnesol, K(i) = 7.33 muM; alpha-Phellandrene, K(i) = 8.71 muM) and two sex pheromone components (Z11-16: OAc, K(i) = 2.84 muM; Z11-16: OH, K(i) = 3.30 muM). These results indicate that OachGOBP1 and OachGOBP2 differ in terms of odorants and other ligands. Furthermore, key amino acid residues that bind to plant volatiles were identified in GOBPs using 3-D structure modeling and ligand molecular docking, predicting the interactions between the GOBPs and the host plant volatiles"
Keywords:		Orthaga achiatina fluorescence competitive binding assay general odorant binding protein molecular docking;
Notes:		"PubMed-not-MEDLINEMa, Yu Li, Yu Wei, Zhi-Qiang Hou, Jing-Hao Si, Yu-Xiao Zhang, Jin Dong, Shuang-Lin Yan, Qi eng 31970455/National Natural Science Foundation of China/ Switzerland 2023/03/29 Insects. 2023 Feb 22; 14(3):216. doi: 10.3390/insects14030216"