| Title: | Activation of the ROS/CncC and 20-Hydroxyecdysone Signaling Pathways Is Associated with Xanthotoxin-Induced Tolerance to lambda-Cyhalothrin in Spodoptera litura |
| Author(s): | Lu K; Li Y; Cheng Y; Li W; Zeng B; Gu C; Zeng R; Song Y; |
| Address: | "State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Adaptation to phytochemicals in herbivorous insects can influence tolerance to insecticides. However, it is unclear how insects use phytochemicals as cues to activate their metabolic detoxification systems. In this study, we found that dietary exposure to xanthotoxin enhanced tolerance of Spodoptera litura larvae to lambda-cyhalothrin. Xanthotoxin ingestion significantly elevated the mRNA levels of 35 detoxification genes as well as the transcription factors Cap 'n' collar isoform-C (CncC) and its binding factor small muscle aponeurosis fibromatosis isoform-K (MafK). Additionally, xanthotoxin exposure increased the levels of reactive oxygen species (ROS), while ROS inhibitor N-acetylcysteine (NAC) treatment blocked xanthotoxin-induced expression of CncC, MafK, and detoxification genes and also prevented xanthotoxin-enhanced larval tolerance to lambda-cyhalothrin. The 20-hydroxyecdysone (20E) signaling pathway was effectively activated by xanthotoxin, while blocking of 20E signaling transduction prevented xanthotoxin-enhanced larval tolerance to lambda-cyhalothrin. Application of 20E induced the expression of multiple xanthotoxin-induced detoxification genes and enhanced lambda-cyhalothrin tolerance in S. litura. NAC treatment blocked xanthotoxin-induced 20E synthesis, while the CncC agonist curcumin activated the 20E signaling pathway. These results indicate that the ROS/CncC pathway controls the induction of metabolic detoxification upon exposure to xanthotoxin, at least in part, through its regulation of the 20E signaling pathway" |
| Keywords: | Animals *Ecdysterone Insect Proteins/metabolism *Insecticides/pharmacology Larva/genetics/metabolism Methoxsalen Nitriles Pyrethrins Reactive Oxygen Species Signal Transduction Spodoptera/genetics/metabolism Spodoptera litura ecdysone insecticide toleranc; |
| Notes: | "MedlineLu, Kai Li, Yimin Cheng, Yibei Li, Wenru Zeng, Bixue Gu, Chengzhen Zeng, Rensen Song, Yuanyuan eng 2021/11/09 J Agric Food Chem. 2021 Nov 17; 69(45):13425-13435. doi: 10.1021/acs.jafc.1c04519. Epub 2021 Nov 8" |
